| Literature DB >> 16216488 |
Kim E Sapsford1, Carissa M Soto, Amy Szuchmacher Blum, Anju Chatterji, Tianwei Lin, John E Johnson, Frances S Ligler, Banahalli R Ratna.
Abstract
Cowpea mosaic virus (CPMV), an icosahedral 30 nm virus, offers a uniquely programmable biological nanoscaffold. This study reports initial optimization of the simultaneous modification of two CPMV mutants with AlexaFluor 647 fluorescent dyes and either IgG proteins or antibodies at specific sites on the virus scaffold. The capacity of CPMV as a simultaneous carrier for different types of molecules was demonstrated, specifically, when applied as a tracer in direct and sandwich immunoassays. The ability to label the virus capsid with antibody and up to 60 fluorescent dyes resulted in an improved limit of detection in SEB sandwich immunoassays, when used as a tracer, relative to a mole equivalent of dye-labeled antibody.Entities:
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Year: 2005 PMID： 16216488 DOI： 10.1016/j.bios.2005.09.003
Source DB: PubMed Journal: Biosens Bioelectron ISSN： 0956-5663 Impact factor: 10.618