Literature DB >> 16216355

A CARD-FISH protocol for the identification and enumeration of epiphytic bacteria on marine algae.

Niina A Tujula1, Carola Holmström, Marc Mussmann, Rudolf Amann, Staffan Kjelleberg, Gregory R Crocetti.   

Abstract

A CARD-FISH protocol was developed and applied to analyse surface-associated bacteria on the marine algae Ulva lactuca, Delisea pulchra, Corallina officinalis, Amphiroa anceps, Porphyra sp. and Sargassum linearifolium. The combination of Alexa(546)-labelled tyramide as the reporter molecule with SYBR Green II counterstain allowed for superior detection of the hybridised probe fluorescence against plant tissue from which pigment autofluorescence has been reduced.

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Year:  2005        PMID： 16216355     DOI： 10.1016/j.mimet.2005.09.006

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  11 in total

1.  Abundance, diversity and activity of sulfate-reducing prokaryotes in heavy metal-contaminated sediment from a salt marsh in the Medway Estuary (UK).

Authors:  Laurent Quillet; Ludovic Besaury; Milka Popova; Sandrine Paissé; Julien Deloffre; Baghdad Ouddane
Journal:  Mar Biotechnol (NY)       Date:  2011-11-30       Impact factor: 3.619

2.  Catalyzed reporter deposition-fluorescence in situ hybridization allows for enrichment-independent detection of microcolony-forming soil bacteria.

Authors:  Belinda C Ferrari; Niina Tujula; Kate Stoner; Staffan Kjelleberg
Journal:  Appl Environ Microbiol       Date:  2006-01       Impact factor: 4.792

3.  Enzymatic permeabilization of the thecate dinoflagellate Alexandrium minutum (Dinophyceae) yields detection of intracellularly associated bacteria via catalyzed reporter deposition-fluorescence in situ hybridization.

Authors:  Lucía Palacios; Irma Marín
Journal:  Appl Environ Microbiol       Date:  2008-02-08       Impact factor: 4.792

4.  Tissue-associated "Candidatus Mycoplasma corallicola" and filamentous bacteria on the cold-water coral Lophelia pertusa (Scleractinia).

Authors:  Sven C Neulinger; Andrea Gärtner; Johanna Järnegren; Martin Ludvigsen; Karin Lochte; Wolf-Christian Dullo
Journal:  Appl Environ Microbiol       Date:  2008-12-29       Impact factor: 4.792

5.  Selective extraction of bacterial DNA from the surfaces of macroalgae.

Authors:  Catherine Burke; Staffan Kjelleberg; Torsten Thomas
Journal:  Appl Environ Microbiol       Date:  2008-10-31       Impact factor: 4.792

6.  Quantitative assessment of ammonia-oxidizing bacterial communities in the epiphyton of submerged macrophytes in shallow lakes.

Authors:  M Coci; G W Nicol; G N Pilloni; M Schmid; M P Kamst-van Agterveld; P L E Bodelier; H J Laanbroek
Journal:  Appl Environ Microbiol       Date:  2010-01-22       Impact factor: 4.792

7.  Quantification of target molecules needed to detect microorganisms by fluorescence in situ hybridization (FISH) and catalyzed reporter deposition-FISH.

Authors:  Tatsuhiko Hoshino; L Safak Yilmaz; Daniel R Noguera; Holger Daims; Michael Wagner
Journal:  Appl Environ Microbiol       Date:  2008-06-13       Impact factor: 4.792

8.  Who is in there? Exploration of endophytic bacteria within the siphonous green seaweed Bryopsis (Bryopsidales, Chlorophyta).

Authors:  Joke Hollants; Olivier Leroux; Frederik Leliaert; Helen Decleyre; Olivier De Clerck; Anne Willems
Journal:  PLoS One       Date:  2011-10-18       Impact factor: 3.240

9.  Microbial and chemical characterization of underwater fresh water springs in the Dead Sea.

Authors:  Danny Ionescu; Christian Siebert; Lubos Polerecky; Yaniv Y Munwes; Christian Lott; Stefan Häusler; Mina Bižić-Ionescu; Christian Quast; Jörg Peplies; Frank Oliver Glöckner; Alban Ramette; Tino Rödiger; Thorsten Dittmar; Aharon Oren; Stefan Geyer; Hans-Joachim Stärk; Martin Sauter; Tobias Licha; Jonathan B Laronne; Dirk de Beer
Journal:  PLoS One       Date:  2012-06-05       Impact factor: 3.240

10.  Identification of Associations between Bacterioplankton and Photosynthetic Picoeukaryotes in Coastal Waters.

Authors:  Hanna M Farnelid; Kendra A Turk-Kubo; Jonathan P Zehr
Journal:  Front Microbiol       Date:  2016-03-22       Impact factor: 5.640
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