Literature DB >> 16208020

Development of a nested qualitative real-time PCR assay to detect Aspergillus species DNA in clinical specimens.

Catriona Halliday1, Qi Xuan Wu, Gregory James, Tania Sorrell.   

Abstract

We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a Light Cycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-intensive. Its use will aid in the rapid diagnosis of invasive aspergillosis.

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Year:  2005        PMID： 16208020      PMCID： PMC1248492          DOI： 10.1128/JCM.43.10.5366-5368.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Diagnosis of invasive aspergillosis in bone marrow transplant recipients by polymerase chain reaction.

Authors:  E C Williamson; J P Leeming; H M Palmer; C G Steward; D Warnock; D I Marks; M R Millar
Journal:  Br J Haematol       Date:  2000-01       Impact factor: 6.998

2.  Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system.

Authors:  J Loeffler; N Henke; H Hebart; D Schmidt; L Hagmeyer; U Schumacher; H Einsele
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

3.  Comparison of real-time PCR, conventional PCR, and galactomannan antigen detection by enzyme-linked immunosorbent assay using bronchoalveolar lavage fluid samples from hematology patients for diagnosis of invasive pulmonary aspergillosis.

Authors:  Maurizio Sanguinetti; Brunella Posteraro; Livio Pagano; Gabriella Pagliari; Luana Fianchi; Luca Mele; Marilena La Sorda; Angelica Franco; Giovanni Fadda
Journal:  J Clin Microbiol       Date:  2003-08       Impact factor: 5.948

4.  Prospective comparison of the diagnostic potential of real-time PCR, double-sandwich enzyme-linked immunosorbent assay for galactomannan, and a (1-->3)-beta-D-glucan test in weekly screening for invasive aspergillosis in patients with hematological disorders.

Authors:  Masahito Kawazu; Yoshinobu Kanda; Yasuhito Nannya; Katsunori Aoki; Mineo Kurokawa; Shigeru Chiba; Toru Motokura; Hisamaru Hirai; Seishi Ogawa
Journal:  J Clin Microbiol       Date:  2004-06       Impact factor: 5.948

5.  Detection of seven Candida species using the Light-Cycler system.

Authors:  P Lewis White; Anjali Shetty; Rosemary A Barnes
Journal:  J Med Microbiol       Date:  2003-03       Impact factor: 2.472

6.  Real-time PCR coupled with automated DNA extraction and detection of galactomannan antigen in serum by enzyme-linked immunosorbent assay for diagnosis of invasive aspergillosis.

Authors:  Catherine Costa; Jean-Marc Costa; Christophe Desterke; Françoise Botterel; Catherine Cordonnier; Stéphane Bretagne
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

7.  Specific detection of Aspergillus species in blood and bronchoalveolar lavage samples of immunocompromised patients by two-step PCR.

Authors:  H Skladny; D Buchheidt; C Baust; F Krieg-Schneider; W Seifarth; C Leib-Mösch; R Hehlmann
Journal:  J Clin Microbiol       Date:  1999-12       Impact factor: 5.948

8.  Use of real-time PCR on blood samples for diagnosis of invasive aspergillosis.

Authors:  M Kami; T Fukui; S Ogawa; Y Kazuyama; U Machida; Y Tanaka; Y Kanda; T Kashima; Y Yamazaki; T Hamaki; S Mori; H Akiyama; Y Mutou; H Sakamaki; K Osumi; S Kimura; H Hirai
Journal:  Clin Infect Dis       Date:  2001-10-04       Impact factor: 9.079

9.  Real-time automated polymerase chain reaction (PCR) to detect Candida albicans and Aspergillus fumigatus DNA in whole blood from high-risk patients.

Authors:  Todd M Pryce; Ian D Kay; Silvano Palladino; Christopher H Heath
Journal:  Diagn Microbiol Infect Dis       Date:  2003-11       Impact factor: 2.803

10.  Development and validation of a quantitative real-time PCR assay using fluorescence resonance energy transfer technology for detection of Aspergillus fumigatus in experimental invasive pulmonary aspergillosis.

Authors:  Cathal E O'Sullivan; Miki Kasai; Andrea Francesconi; Vidmantas Petraitis; Ruta Petraitiene; Amy M Kelaher; Alia A Sarafandi; Thomas J Walsh
Journal:  J Clin Microbiol       Date:  2003-12       Impact factor: 5.948
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  10 in total

1.  Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water.

Authors:  Nicolas Radomski; Françoise S Lucas; Régis Moilleron; Emmanuelle Cambau; Sophie Haenn; Laurent Moulin
Journal:  Appl Environ Microbiol       Date:  2010-09-17       Impact factor: 4.792

2.  A novel extraction method combining plasma with a whole-blood fraction shows excellent sensitivity and reproducibility for patients at high risk for invasive aspergillosis.

Authors:  Jan Springer; Hannes Schloßnagel; Werner Heinz; Thomas Doedt; Rainer Soeller; Hermann Einsele; Juergen Loeffler
Journal:  J Clin Microbiol       Date:  2012-05-16       Impact factor: 5.948

3.  Quantification of nitrogen reductase and nitrite reductase genes in soil of thinned and clear-cut Douglas-fir stands by using real-time PCR.

Authors:  David J Levy-Booth; Richard S Winder
Journal:  Appl Environ Microbiol       Date:  2010-08-27       Impact factor: 4.792

4.  Diagnosis of invasive aspergillosis using bronchoalveolar lavage in haematology patients: influence of bronchoalveolar lavage human DNA content on real-time PCR performance.

Authors:  E Fréalle; K Decrucq; F Botterel; B Bouchindhomme; D Camus; E Dei-Cas; J M Costa; I Yakoub-Agha; S Bretagne; L Delhaes
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-09-02       Impact factor: 3.267

5.  Highly sensitive methods based on seminested real-time reverse transcription-PCR for quantitation of human immunodeficiency virus type 1 unspliced and multiply spliced RNA and proviral DNA.

Authors:  Alexander O Pasternak; Karen W Adema; Margreet Bakker; Suzanne Jurriaans; Ben Berkhout; Marion Cornelissen; Vladimir V Lukashov
Journal:  J Clin Microbiol       Date:  2008-05-07       Impact factor: 5.948

6.  Reverse line blot hybridization assay for identification of medically important fungi from culture and clinical specimens.

Authors:  Xianyu Zeng; Fanrong Kong; Catriona Halliday; Sharon Chen; Anna Lau; Geoffrey Playford; Tania C Sorrell
Journal:  J Clin Microbiol       Date:  2007-07-18       Impact factor: 5.948

7.  A MIQE-compliant real-time PCR assay for Aspergillus detection.

Authors:  Gemma L Johnson; David F Bibby; Stephenie Wong; Samir G Agrawal; Stephen A Bustin
Journal:  PLoS One       Date:  2012-07-10       Impact factor: 3.240

8.  Polymerase chain reaction blood tests for the diagnosis of invasive aspergillosis in immunocompromised people.

Authors:  Mario Cruciani; Carlo Mengoli; Rosemary Barnes; J Peter Donnelly; Juergen Loeffler; Brian L Jones; Lena Klingspor; Johan Maertens; Charles O Morton; Lewis P White
Journal:  Cochrane Database Syst Rev       Date:  2019-09-03

9.  Development of a novel inhalational model of invasive pulmonary aspergillosis in rats and comparative evaluation of three biomarkers for its diagnosis.

Authors:  Suhail Ahmad; Ali A Al-Shaikh; Ziauddin Khan
Journal:  PLoS One       Date:  2014-06-23       Impact factor: 3.240

Review 10.  Laboratory diagnosis of invasive aspergillosis: from diagnosis to prediction of outcome.

Authors:  Richard C Barton
Journal:  Scientifica (Cairo)       Date:  2013-01-14
  10 in total

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