Literature DB >> 1619658

Crystallization of Sindbis virus and its nucleocapsid.

S C Harrison1, R K Strong, S Schlesinger, M J Schlesinger.   

Abstract

Crystals of Sindbis virus, which contains a lipid-bilayer membrane, have been grown using polyethylene glycol. The space group is R32, a = b = 640 A, c = 1520 A. The crystals are highly mosaic, and recorded diffraction is therefore restricted to spacings of about 30 A. The crystals show that the packing of glycoproteins E1 and E2 in the icosahedral outer shell is sufficiently precise that it permits regular and repeated interactions between virus particles in the lattice. Crystals of Sindbis nucleocapsids have also been grown. The limited diffraction data are consistent with close packing of nucleocapsids 404 A in diameter.

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Year:  1992        PMID: 1619658     DOI: 10.1016/0022-2836(92)90141-6

Source DB:  PubMed          Journal:  J Mol Biol        ISSN: 0022-2836            Impact factor:   5.469


  16 in total

1.  Locations of carbohydrate sites on alphavirus glycoproteins show that E1 forms an icosahedral scaffold.

Authors:  Sergei V Pletnev; Wei Zhang; Suchetana Mukhopadhyay; Bonnie R Fisher; Raquel Hernandez; Dennis T Brown; Timothy S Baker; Michael G Rossmann; Richard J Kuhn
Journal:  Cell       Date:  2001-04-06       Impact factor: 41.582

2.  In vitro assembly of Sindbis virus core-like particles from cross-linked dimers of truncated and mutant capsid proteins.

Authors:  T L Tellinghuisen; R Perera; R J Kuhn
Journal:  J Virol       Date:  2001-03       Impact factor: 5.103

Review 3.  Virus crystallography.

Authors:  E E Fry; J Grimes; D I Stuart
Journal:  Mol Biotechnol       Date:  1999-08       Impact factor: 2.695

4.  Crystallization and preliminary X-ray diffraction analysis of West Nile virus.

Authors:  Bärbel Kaufmann; Pavel Plevka; Richard J Kuhn; Michael G Rossmann
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-04-30

5.  Putative receptor binding sites on alphaviruses as visualized by cryoelectron microscopy.

Authors:  T J Smith; R H Cheng; N H Olson; P Peterson; E Chase; R J Kuhn; T S Baker
Journal:  Proc Natl Acad Sci U S A       Date:  1995-11-07       Impact factor: 11.205

6.  Nucleocapsid and glycoprotein organization in an enveloped virus.

Authors:  R H Cheng; R J Kuhn; N H Olson; M G Rossmann; H K Choi; T J Smith; T S Baker
Journal:  Cell       Date:  1995-02-24       Impact factor: 41.582

7.  In vitro assembly of alphavirus cores by using nucleocapsid protein expressed in Escherichia coli.

Authors:  T L Tellinghuisen; A E Hamburger; B R Fisher; R Ostendorp; R J Kuhn
Journal:  J Virol       Date:  1999-07       Impact factor: 5.103

Review 8.  The alphaviruses: gene expression, replication, and evolution.

Authors:  J H Strauss; E G Strauss
Journal:  Microbiol Rev       Date:  1994-09

9.  Aura virus structure suggests that the T=4 organization is a fundamental property of viral structural proteins.

Authors:  Wei Zhang; Bonnie R Fisher; Norman H Olson; James H Strauss; Richard J Kuhn; Timothy S Baker
Journal:  J Virol       Date:  2002-07       Impact factor: 5.103

10.  The 6-kilodalton membrane protein of Semliki Forest virus is involved in the budding process.

Authors:  A Loewy; J Smyth; C H von Bonsdorff; P Liljeström; M J Schlesinger
Journal:  J Virol       Date:  1995-01       Impact factor: 5.103

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