Rohini Edirisinghe1, Rodney Jemmott, John Allan. 1. The Wesley IVF Service, The Wesley Hospital, Auchenflower, Brisbane, Queensland, Australia. Rohini.Edirisinghe@wesley.com.au
Abstract
PURPOSE: To report a comparison of growth rates between fresh and frozen-thawed embryos on day 3 and 4 according to chromosomal status during PGD. METHODS: Embryos were biopsied on day 3, fluorescent in situ hybridization performed for aneuploidy detection and growth rates monitored. RESULTS: Based on 241 egg pick up-PGD and 133 frozen embryo transfer-PGD cycles. The development rate of embryos despite their chromosomal status was similar on day 3, however, overall, fresh embryos showed a significantly improved development (> or =6 cell stage; 85.7%) compared to frozen embryos (77.0%; p < 0.003). On day 4 fresh embryos showed accelerated development and further developmental improvements were associated with chromosomal normality (56.1% normal vs. 42.2% aneuploid; p < 0.004). CONCLUSIONS: Cryopreservation slows embryo development regardless of the chromosomal status. However, chromosomal status did not influence the chance of embryo implantation which was the same for fresh and thawed embryos.
PURPOSE: To report a comparison of growth rates between fresh and frozen-thawed embryos on day 3 and 4 according to chromosomal status during PGD. METHODS: Embryos were biopsied on day 3, fluorescent in situ hybridization performed for aneuploidy detection and growth rates monitored. RESULTS: Based on 241 egg pick up-PGD and 133 frozen embryo transfer-PGD cycles. The development rate of embryos despite their chromosomal status was similar on day 3, however, overall, fresh embryos showed a significantly improved development (> or =6 cell stage; 85.7%) compared to frozen embryos (77.0%; p < 0.003). On day 4 fresh embryos showed accelerated development and further developmental improvements were associated with chromosomal normality (56.1% normal vs. 42.2% aneuploid; p < 0.004). CONCLUSIONS: Cryopreservation slows embryo development regardless of the chromosomal status. However, chromosomal status did not influence the chance of embryo implantation which was the same for fresh and thawed embryos.
Authors: John J Orris; Tyl H Taylor; Janice W Gilchrist; Susan V Hallowell; Michael J Glassner; J David Wininger Journal: J Assist Reprod Genet Date: 2010-09-02 Impact factor: 3.412