Literature DB >> 16190707

Efficient strategy for the rapid backbone assignment of membrane proteins.

Nikola Trbovic1, Christian Klammt, Alexander Koglin, Frank Löhr, Frank Bernhard, Volker Dötsch.   

Abstract

Investigations of membrane proteins pose one of the biggest current challenges in structural biology. Recent advances in protein production techniques based on cell-free transcription/translation methods have, however, opened new opportunities in this area. Here, we report an efficient protocol for the backbone assignment of membrane proteins as the first step of NMR-based structure determination.

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Year:  2005        PMID： 16190707     DOI： 10.1021/ja0540270

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  22 in total

1.  Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins.

Authors:  Hak Jun Kim; Stanley C Howell; Wade D Van Horn; Young Ho Jeon; Charles R Sanders
Journal:  Prog Nucl Magn Reson Spectrosc       Date:  2009-11-01       Impact factor: 9.795

2.  Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis.

Authors:  Innokentiy Maslennikov; Christian Klammt; Eunha Hwang; Georgia Kefala; Mizuki Okamura; Luis Esquivies; Karsten Mörs; Clemens Glaubitz; Witek Kwiatkowski; Young Ho Jeon; Senyon Choe
Journal:  Proc Natl Acad Sci U S A       Date:  2010-05-24       Impact factor: 11.205

3.  Stable isotope labeling strategy based on coding theory.

Authors:  Takuma Kasai; Seizo Koshiba; Jun Yokoyama; Takanori Kigawa
Journal:  J Biomol NMR       Date:  2015-08-21       Impact factor: 2.835

4.  Amino-acid type identification in 15N-HSQC spectra by combinatorial selective 15N-labelling.

Authors:  Peter S C Wu; Kiyoshi Ozawa; Slobodan Jergic; Xun-Cheng Su; Nicholas E Dixon; Gottfried Otting
Journal:  J Biomol NMR       Date:  2006-01       Impact factor: 2.835

5.  Increasing the accuracy of solution NMR structures of membrane proteins by application of residual dipolar couplings. High-resolution structure of outer membrane protein A.

Authors:  Tomasz Cierpicki; Binyong Liang; Lukas K Tamm; John H Bushweller
Journal:  J Am Chem Soc       Date:  2006-05-31       Impact factor: 15.419

Review 6.  Solution NMR of membrane proteins in bilayer mimics: small is beautiful, but sometimes bigger is better.

Authors:  Sébastien F Poget; Mark E Girvin
Journal:  Biochim Biophys Acta       Date:  2007-09-20

7.  Transmembrane segment enhanced labeling as a tool for the backbone assignment of alpha-helical membrane proteins.

Authors:  Sina Reckel; Solmaz Sobhanifar; Birgit Schneider; Friederike Junge; Daniel Schwarz; Florian Durst; Frank Löhr; Peter Güntert; Frank Bernhard; Volker Dötsch
Journal:  Proc Natl Acad Sci U S A       Date:  2008-06-11       Impact factor: 11.205

8.  Cell-free expression and stable isotope labelling strategies for membrane proteins.

Authors:  Solmaz Sobhanifar; Sina Reckel; Friederike Junge; Daniel Schwarz; Lei Kai; Mikhail Karbyshev; Frank Löhr; Frank Bernhard; Volker Dötsch
Journal:  J Biomol NMR       Date:  2009-08-13       Impact factor: 2.835

9.  Isotope labeling of mammalian GPCRs in HEK293 cells and characterization of the C-terminus of bovine rhodopsin by high resolution liquid NMR spectroscopy.

Authors:  Karla Werner; Christian Richter; Judith Klein-Seetharaman; Harald Schwalbe
Journal:  J Biomol NMR       Date:  2007-11-13       Impact factor: 2.835

10.  Carbonyl carbon label selective (CCLS) 1H-15N HSQC experiment for improved detection of backbone 13C-15N cross peaks in larger proteins.

Authors:  Marco Tonelli; Larry R Masterson; Klaas Hallenga; Gianluigi Veglia; John L Markley
Journal:  J Biomol NMR       Date:  2007-09-09       Impact factor: 2.835
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