Literature DB >> 16188439

Photoregulation of DNA polymerase I (Klenow) with caged fluorescent oligodeoxynucleotides.

XinJing Tang1, Julia L Richards, Adam E Peritz, Ivan J Dmochowski.   

Abstract

The DNA polymerase reaction by Klenow fragment (KF) was efficiently regulated with UV light using a 25-mer caged fluorescent oligodeoxynucleotide (CFO) as the template. The CFO was functionalized with a fluorescein reporter (Fl) and photocleavable DABSYL quencher moiety (Dab). With Fl and Dab at adjacent cytidines in the middle at the template, KF was blocked from extending a complementary 12-mer primer. Upon UV photolysis of the DABSYL blocking group under aerobic conditions, fluorescein emission was restored and 50% of the primers were fully extended by KF.

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Year:  2005        PMID： 16188439     DOI： 10.1016/j.bmcl.2005.08.058

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  10 in total

1.  Light-activated deoxyguanosine: photochemical regulation of peroxidase activity.

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Journal:  Mol Biosyst       Date:  2008-04-08

Review 2.  Photoremovable protecting groups in chemistry and biology: reaction mechanisms and efficacy.

Authors:  Petr Klán; Tomáš Šolomek; Christian G Bochet; Aurélien Blanc; Richard Givens; Marina Rubina; Vladimir Popik; Alexey Kostikov; Jakob Wirz
Journal:  Chem Rev       Date:  2012-12-21       Impact factor: 60.622

3.  Mismatch Discrimination and Efficient Photomodulation with Split 10-23 DNAzymes.

Authors:  Brittani K Ruble; Julia L Richards; Jasmina C Cheung-Lau; Ivan J Dmochowski
Journal:  Inorganica Chim Acta       Date:  2012-01-15       Impact factor: 2.545

4.  Photoactivatable Circular Caged Oligonucleotides for Transcriptome In Vivo Analysis (TIVA).

Authors:  Linlin Yang; Dora von Trentini; HyunBum Kim; Jai-Yoon Sul; James H Eberwine; Ivan J Dmochowski
Journal:  ChemPhotoChem       Date:  2021-06-23

5.  RNA bandages for photoregulating in vitro protein synthesis.

Authors:  Julia L Richards; XinJing Tang; Anna Turetsky; Ivan J Dmochowski
Journal:  Bioorg Med Chem Lett       Date:  2008-09-30       Impact factor: 2.823

6.  Optochemical control of deoxyoligonucleotide function via a nucleobase-caging approach.

Authors:  Qingyang Liu; Alexander Deiters
Journal:  Acc Chem Res       Date:  2013-08-28       Impact factor: 22.384

7.  Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization.

Authors:  Douglas D Young; Hrvoje Lusic; Mark O Lively; Alexander Deiters
Journal:  Nucleic Acids Res       Date:  2009-03-17       Impact factor: 16.971

8.  Light-activated RNA interference using double-stranded siRNA precursors modified using a remarkable regiospecificity of diazo-based photolabile groups.

Authors:  Samit Shah; Piyush K Jain; Ashish Kala; Dipu Karunakaran; Simon H Friedman
Journal:  Nucleic Acids Res       Date:  2009-05-28       Impact factor: 16.971

9.  Manipulation of gene expression in zebrafish using caged circular morpholino oligomers.

Authors:  Yuan Wang; Li Wu; Peng Wang; Cong Lv; Zhenjun Yang; Xinjing Tang
Journal:  Nucleic Acids Res       Date:  2012-09-22       Impact factor: 16.971

Review 10.  Conditionally Activated ("Caged") Oligonucleotides.

Authors:  Linlin Yang; Ivan J Dmochowski
Journal:  Molecules       Date:  2021-03-09       Impact factor: 4.411
  10 in total

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