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Literature DB >> 1618326

Acidic pH induces fusion of cells infected with baculovirus to form syncytia.

Abstract

The enveloped baculovirus insect cell system has been used extensively for expression of recombinant proteins, including viral fusion proteins. We tested wild-type baculovirus for endogenous fusion protein activity. Syncytia formation, dye transfer, and capacitance changes were observed after incubating infected Spodoptera frugiperda cells in acidic media, consistent with fusion protein activity. Only a short acidic pulse of 10 s is needed to trigger syncytia formation. Identical results were obtained with recombinant baculovirus. This new system is convenient for studying pH activated cell-cell fusion. However, using this enveloped virus to study the mechanism of recombinant fusion proteins requires caution.

Entities: Disease Species

Mesh：

Substances：
Acids

Year: 1992 PMID： 1618326 PMCID： PMC7130246 DOI： 10.1016/0014-5793(92)80623-o

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

11 in total

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10. Acidic pH induces fusion of cells infected with baculovirus to form syncytia.

Authors: E Leikina; H O Onaran; J Zimmerberg
Journal: FEBS Lett Date: 1992-06-15 Impact factor: 4.124

26 in total

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Authors: Ilya Plonsky; David H Kingsley; Afshin Rashtian; Paul S Blank; Joshua Zimmerberg
Journal: Biol Cell Date: 2008-06 Impact factor: 4.458

3. The pre-transmembrane domain of the Autographa californica multicapsid nucleopolyhedrovirus GP64 protein is critical for membrane fusion and virus infectivity.

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Journal: J Virol Date: 2009-08-19 Impact factor: 5.103

4. Persistent gene expression in mouse nasal epithelia following feline immunodeficiency virus-based vector gene transfer.

Authors: Patrick L Sinn; Erin R Burnight; Melissa A Hickey; Gary W Blissard; Paul B McCray
Journal: J Virol Date: 2005-10 Impact factor: 5.103

5. Autographa californica multicapsid nucleopolyhedrovirus efficiently infects Sf9 cells and transduces mammalian cells via direct fusion with the plasma membrane at low pH.

Authors: Sicong Dong; Manli Wang; Zhijuan Qiu; Fei Deng; Just M Vlak; Zhihong Hu; Hualin Wang
Journal: J Virol Date: 2010-03-10 Impact factor: 5.103

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Journal: J Virol Date: 2007-11-07 Impact factor: 5.103

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Journal: J Virol Date: 1995-05 Impact factor: 5.103

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Authors: Feifei Yin; Manli Wang; Ying Tan; Fei Deng; Just M Vlak; Zhihong Hu; Hualin Wang
Journal: J Virol Date: 2008-06-18 Impact factor: 5.103