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Literature DB >> 1617735

High efficiency transformation of Fusarium solani f. sp. cucurbitae race 2 (mating population V).

R N Crowhurst¹, J Rees-George, E H Rikkerink, M D Templeton.

Abstract

A cosmid vector, suitable for library construction and DNA transformation in filamentous fungi, has been constructed and a reliable and highly efficient PEG-mediated DNA transformation system for F. solani f. sp. cucurbitae, based on resistance to hygromycin B, has been developed for use with this vector. This transformation system yielded 10(4) transformants per micrograms of DNA when using 10(7) protoplasts. Factors important in achieving high efficiency included: the maintenance of an osmoticum in all transformation steps, PEG 4000 concentration, and the ratio of transforming vector DNA to protoplasts. Approximately 60% of transformants stably integrated vector DNA. Molecular analysis revealed multiple copies of the plasmid integrated into the genome at one or more sites. The frequency of transformation achieved will facilitate the isolation of genes from this fungus by complementation.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1992 PMID： 1617735 DOI： 10.1007/bf00351656

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

12 in total

1. Gene transfer system for the phytopathogenic fungus Ustilago maydis.

Authors: J Wang; D W Holden; S A Leong
Journal: Proc Natl Acad Sci U S A Date: 1988-02 Impact factor: 11.205

2. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.

Authors: P J Punt; R P Oliver; M A Dingemanse; P H Pouwels; C A van den Hondel
Journal: Gene Date: 1987 Impact factor: 3.688

3. Molecular transformation of Fusarium solani with an antibiotic resistance marker having no fungal DNA homology.

Authors: E T Marek; C L Schardl; D A Smith
Journal: Curr Genet Date: 1989-06 Impact factor: 3.886

4. Transformation of seven species of filamentous fungi using the nitrate reductase gene of Aspergillus nidulans.

Authors: M J Daboussi; A Djeballi; C Gerlinger; P L Blaiseau; I Bouvier; M Cassan; M H Lebrun; D Parisot; Y Brygoo
Journal: Curr Genet Date: 1989-06 Impact factor: 3.886

5. High efficiency vectors for cosmid microcloning and genomic analysis.

Authors: G A Evans; K Lewis; B E Rothenberg
Journal: Gene Date: 1989-06-30 Impact factor: 3.688

6. Cloning large segments of genomic DNA using cosmid vectors.

Authors: A G DiLella; S L Woo
Journal: Methods Enzymol Date: 1987 Impact factor: 1.600

7. Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase.

Authors: P J Punt; M A Dingemanse; A Kuyvenhoven; R D Soede; P H Pouwels; C A van den Hondel
Journal: Gene Date: 1990-09-01 Impact factor: 3.688

3. Bistability and hysteresis of the 'Secteur' differentiation are controlled by a two-gene locus in Nectria haematococca.

Authors: Stéphane Graziani; Philippe Silar; Marie-Josée Daboussi
Journal: BMC Biol Date: 2004-08-16 Impact factor: 7.431

3 in total

High efficiency transformation of Fusarium solani f. sp. cucurbitae race 2 (mating population V).

1. Gene transfer system for the phytopathogenic fungus Ustilago maydis.

2. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.

3. Molecular transformation of Fusarium solani with an antibiotic resistance marker having no fungal DNA homology.

4. Transformation of seven species of filamentous fungi using the nitrate reductase gene of Aspergillus nidulans.

5. High efficiency vectors for cosmid microcloning and genomic analysis.

6. Cloning large segments of genomic DNA using cosmid vectors.

7. Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase.

8. Efficient cloning of genes of Neurospora crassa.

9. Transformation of Neurospora crassa with circular and linear DNA and analysis of the fate of the transforming DNA.

10. Differentiation of Fusarium solani f. sp. cucurbitae races 1 and 2 by random amplification of polymorphic DNA.

Review 1. Advances in linking polyketides and non-ribosomal peptides to their biosynthetic gene clusters in Fusarium.

2. A homologous and self-replicating system for efficient transformation of Fusarium oxysporum.

3. Bistability and hysteresis of the 'Secteur' differentiation are controlled by a two-gene locus in Nectria haematococca.