In vitro propagation of rose--a review.

In vitro propagation of rose has played a very important role in rapid multiplication of cultivars with desirable traits and production of healthy and disease-free plants. During the last several years, different approaches have been made for in vitro propagation of rose. Micropropagation using apical buds or nodal segments and understanding the specific requirements at different stages has been comprehensively covered in literature. New challenges for refinements of protocols for high rate of shoot multiplication and development of cost effective methods has gained importance in the recent past. Importance of liquid static culture for shoot proliferation and root induction for rose is also discussed in the present review. Further, the development of protocol for in vitro plant regeneration which is considered as most important step for successful implementation of various biotechnological techniques used for plant improvement programmes has been adequately addressed in literature. In rose, there are several reports which indicate rapid regeneration and multiplication through organogenesis or somatic embryogenesis. On the whole, the present review gives a consolidated account of in vitro propagation in rose.