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Literature DB >> 16163454

Essential cysteine residues in Bacillus subtilis spore photoproduct lyase identified by alanine scanning mutagenesis.

Patricia Fajardo-Cavazos¹, Roberto Rebeil, Wayne L Nicholson.

Abstract

Endospore-forming bacteria (Bacillus and Clostridium spp.) are highly ultraviolet (UV) resistant and repair UV-induced DNA damage in part using the spore-specific DNA repair enzyme spore photoproduct (SP) lyase. SP lyase in all known sporeformers contains four conserved cysteine residues; three absolutely conserved residues are located at the "Radical SAM" consensus (C91xxxC95xxC98), which presumably participates in [4Fe-4S] cluster formation. A fourth conserved cysteine, the function of which is unknown, is located at C141 in SP lyase from all Bacillus spp. sequenced to date. To probe the function of the fourth cysteine, each conserved cysteine in the B. subtilis SP lyase was systematically altered to alanine by site-directed mutagenesis. UV-visible spectroscopy of wild-type and mutant SP lyases indicated that C141 does not participate in [4Fe-4S] formation and redox chemistry; however, in vivo SP lyase activity was abolished in all mutants, indicating an essential role for C141 in SP lyase activity.

Entities: Chemical Species

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Year: 2005 PMID： 16163454 DOI： 10.1007/s00284-005-0052-8

Source DB: PubMed Journal: Curr Microbiol ISSN： 0343-8651 Impact factor: 2.188

23 in total

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Journal: Environ Mol Mutagen Date: 2001 Impact factor: 3.216

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Journal: Annu Rev Microbiol Date: 1988 Impact factor: 15.500

3. Direct H atom abstraction from spore photoproduct C-6 initiates DNA repair in the reaction catalyzed by spore photoproduct lyase: evidence for a reversibly generated adenosyl radical intermediate.

Authors: Jennifer Cheek; Joan B Broderick
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Authors: J Nölling; G Breton; M V Omelchenko; K S Makarova; Q Zeng; R Gibson; H M Lee; J Dubois; D Qiu; J Hitti; Y I Wolf; R L Tatusov; F Sabathe; L Doucette-Stamm; P Soucaille; M J Daly; G N Bennett; E V Koonin; D R Smith
Journal: J Bacteriol Date: 2001-08 Impact factor: 3.490

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Authors: T A Slieman; R Rebeil; W L Nicholson
Journal: J Bacteriol Date: 2000-11 Impact factor: 3.490

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Authors: P Fajardo-Cavazos; C Salazar; W L Nicholson
Journal: J Bacteriol Date: 1993-03 Impact factor: 3.490

7. The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1.

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Journal: Nucleic Acids Res Date: 2004-02-11 Impact factor: 16.971

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Journal: Annu Rev Microbiol Date: 1995 Impact factor: 15.500

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Authors: D Hanahan
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Journal: Genome Biol Date: 2004-09-13 Impact factor: 13.583

15 in total

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Authors: Alexia Chandor-Proust; Olivier Berteau; Thierry Douki; Didier Gasparutto; Sandrine Ollagnier-de-Choudens; Marc Fontecave; Mohamed Atta
Journal: J Biol Chem Date: 2008-10-27 Impact factor: 5.157

2. Probing the reaction mechanism of spore photoproduct lyase (SPL) via diastereoselectively labeled dinucleotide SP TpT substrates.

Authors: Linlin Yang; Gengjie Lin; Degang Liu; Karl J Dria; Joshua Telser; Lei Li
Journal: J Am Chem Soc Date: 2011-06-14 Impact factor: 15.419

Review 3. Radical S-adenosylmethionine enzymes.

Authors: Joan B Broderick; Benjamin R Duffus; Kaitlin S Duschene; Eric M Shepard
Journal: Chem Rev Date: 2014-01-29 Impact factor: 60.622

4. Anaerobic sulfatase-maturating enzyme--a mechanistic link with glycyl radical-activating enzymes?

Authors: Alhosna Benjdia; Sowmya Subramanian; Jérôme Leprince; Hubert Vaudry; Michael K Johnson; Olivier Berteau
Journal: FEBS J Date: 2010-03-09 Impact factor: 5.542

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Authors: Lei Li
Journal: Biochim Biophys Acta Date: 2011-12-08

6. Spore photoproduct within DNA is a surprisingly poor substrate for its designated repair enzyme-The spore photoproduct lyase.

Authors: Linlin Yang; Yajun Jian; Peter Setlow; Lei Li
Journal: DNA Repair (Amst) Date: 2017-03-06