Literature DB >> 16160825

Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy.

Stefan K Kufer1, Hendrik Dietz, Christian Albrecht, Kerstin Blank, Angelika Kardinal, Matthias Rief, Hermann E Gaub.   

Abstract

A genetically modified form of the human DNA repair protein O(6)-alkylguanine-DNA-alkyltransferase (hAGT) was used to immobilize different recombinant hAGT fusion proteins covalently and selectively on gold and glass surfaces. Fusion proteins of hAGT with Glutathione S-Transferase and with tandem repeats of Titin Ig-domains, were produced and anchored via amino-polyethylene glycol benzylguanine. Anchoring was characterized and quantified with surface plasmon resonance, atomic force microscope and fluorescence measurements. Individual fusion proteins were unfolded by single molecule force spectroscopy corroborating the selectivity of the covalent attachment.

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Year:  2005        PMID： 16160825     DOI： 10.1007/s00249-005-0010-1

Source DB:  PubMed          Journal:  Eur Biophys J        ISSN： 0175-7571            Impact factor:   1.733


  11 in total

1.  How strong is a covalent bond?

Authors: 
Journal:  Science       Date:  1999-03-12       Impact factor: 47.728

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Authors:  D S Daniels; J A Tainer
Journal:  Mutat Res       Date:  2000-08-30       Impact factor: 2.433

3.  A general method for the covalent labeling of fusion proteins with small molecules in vivo.

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4.  Directed evolution of O6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo.

Authors:  Alexandre Juillerat; Thomas Gronemeyer; Antje Keppler; Susanne Gendreizig; Horst Pick; Horst Vogel; Kai Johnsson
Journal:  Chem Biol       Date:  2003-04

5.  Covalent and selective immobilization of fusion proteins.

Authors:  Maik Kindermann; Nathalie George; Nils Johnsson; Kai Johnsson
Journal:  J Am Chem Soc       Date:  2003-07-02       Impact factor: 15.419

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Authors:  Hendrik Dietz; Matthias Rief
Journal:  Proc Natl Acad Sci U S A       Date:  2004-11-05       Impact factor: 11.205

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Authors:  M Rief; M Gautel; F Oesterhelt; J M Fernandez; H E Gaub
Journal:  Science       Date:  1997-05-16       Impact factor: 47.728

8.  Active and alkylated human AGT structures: a novel zinc site, inhibitor and extrahelical base binding.

Authors:  D S Daniels; C D Mol; A S Arvai; S Kanugula; A E Pegg; J A Tainer
Journal:  EMBO J       Date:  2000-04-03       Impact factor: 11.598

9.  Mechanism of inactivation of human O6-alkylguanine-DNA alkyltransferase by O6-benzylguanine.

Authors:  A E Pegg; M Boosalis; L Samson; R C Moschel; T L Byers; K Swenn; M E Dolan
Journal:  Biochemistry       Date:  1993-11-16       Impact factor: 3.162

10.  Induced protein dimerization in vivo through covalent labeling.

Authors:  Susanne Gendreizig; Maik Kindermann; Kai Johnsson
Journal:  J Am Chem Soc       Date:  2003-12-10       Impact factor: 15.419
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  14 in total

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Review 5.  Atomic force microscopy with sub-picoNewton force stability for biological applications.

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7.  DNA origami as biocompatible surface to match single-molecule and ensemble experiments.

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Review 8.  Superglue from bacteria: unbreakable bridges for protein nanotechnology.

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9.  GFP's mechanical intermediate states.

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10.  A polypeptide-DNA hybrid with selective linking capability applied to single molecule nano-mechanical measurements using optical tweezers.

Authors:  Fatemeh Moayed; Alireza Mashaghi; Sander J Tans
Journal:  PLoS One       Date:  2013-01-15       Impact factor: 3.240
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