Literature DB >> 16159249

Multicolor imaging of cell surface proteins.

Laura Vivero-Pol1, Nathalie George, Holger Krumm, Kai Johnsson, Nils Johnsson.   

Abstract

We report on a method for the multicolor imaging of cell surface proteins which is based on the labeling of carrier protein (CP) fusion proteins with different fluorophores. In one application, different generations of a cell surface protein can be sequentially labeled to discriminate between old and newly made copies. In another application, fusions to different CPs can be selectively labeled with different fluorophores in one sample. Both applications open up new ways for studying the properties of cell surface proteins of living cells.

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Year:  2005        PMID： 16159249     DOI： 10.1021/ja0533850

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  21 in total

1.  Distribution of resting and ligand-bound ErbB1 and ErbB2 receptor tyrosine kinases in living cells using number and brightness analysis.

Authors:  Peter Nagy; Jeroen Claus; Thomas M Jovin; Donna J Arndt-Jovin
Journal:  Proc Natl Acad Sci U S A       Date:  2010-09-02       Impact factor: 11.205

Review 2.  The phosphopantetheinyl transferases: catalysis of a post-translational modification crucial for life.

Authors:  Joris Beld; Eva C Sonnenschein; Christopher R Vickery; Joseph P Noel; Michael D Burkart
Journal:  Nat Prod Rep       Date:  2014-01       Impact factor: 13.423

3.  Spying on Neuronal Membrane Potential with Genetically Targetable Voltage Indicators.

Authors:  Vincent Grenier; Brittany R Daws; Pei Liu; Evan W Miller
Journal:  J Am Chem Soc       Date:  2019-01-10       Impact factor: 15.419

4.  An eight residue fragment of an acyl carrier protein suffices for post-translational introduction of fluorescent pantetheinyl arms in protein modification in vitro and in vivo.

Authors:  Zhe Zhou; Alexander Koglin; Yu Wang; Andrew P McMahon; Christopher T Walsh
Journal:  J Am Chem Soc       Date:  2008-07-02       Impact factor: 15.419

5.  Breaking the color barrier - a multi-selective antibody reporter offers innovative strategies of fluorescence detection.

Authors:  Eugenio Gallo; Jonathan W Jarvik
Journal:  J Cell Sci       Date:  2017-06-14       Impact factor: 5.285

6.  Efficient and wash-free labeling of membrane proteins using engineered Npu DnaE split-inteins.

Authors:  Euiyeon Lee; Kyoungmi Min; Young-Tae Chang; Youngeun Kwon
Journal:  Protein Sci       Date:  2018-09-24       Impact factor: 6.725

7.  Rapidly rendering cells phagocytic through a cell surface display technique and concurrent Rac activation.

Authors:  Hiroki Onuma; Toru Komatsu; Makoto Arita; Kenjiro Hanaoka; Tasuku Ueno; Takuya Terai; Tetsuo Nagano; Takanari Inoue
Journal:  Sci Signal       Date:  2014-07-15       Impact factor: 8.192

Review 8.  Advances in chemical labeling of proteins in living cells.

Authors:  Qi Yan; Marcel P Bruchez
Journal:  Cell Tissue Res       Date:  2015-03-07       Impact factor: 5.249

9.  Phage display evolution of a peptide substrate for yeast biotin ligase and application to two-color quantum dot labeling of cell surface proteins.

Authors:  Irwin Chen; Yoon-Aa Choi; Alice Y Ting
Journal:  J Am Chem Soc       Date:  2007-05-02       Impact factor: 15.419

10.  A biocompatible condensation reaction for the labeling of terminal cysteine residues on proteins.

Authors:  Hongjun Ren; Fei Xiao; Ke Zhan; Young-Pil Kim; Hexin Xie; Zuyong Xia; Jianghong Rao
Journal:  Angew Chem Int Ed Engl       Date:  2009       Impact factor: 15.336
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