BACKGROUND: Interkeukin-1 (IL-1) gene cluster polymorphisms that are thought to enhance the production of IL-1beta are associated with an increased risk of gastric cancer. To determine the role of host genetic factors in Helicobacter pylori infection, we examined the relationship between gastric mucosal IL-1beta levels and IL-1B polymorphisms in patients with H. pylori infection. METHODS: Biopsy tissues obtained from 99 patients were homogenized and gastric mucosal IL-1beta levels were measured by enzyme-linked immunosorbent assay (ELISA). Single-base polymorphisms at positions -511 and -31 in IL-1B were analyzed. RESULTS: The IL-1beta level in the antrum was significantly higher in genotype IL-1B-511C/C than in H. pylori-negative patients (P < 0.05). The IL-1B polymorphism did not influence the degree of gastric neutrophil and mononuclear cell infiltration, or gastric atrophy. IL-1beta levels in the corpus, but not those in the antrum, correlated to the severity of gastric atrophy. CONCLUSIONS: These findings indicate that IL-1B polymorphisms enhance IL-1beta production in the antrum; however, other factors might regulate the production of IL-1beta in the corpus of the stomach, regardless of IL-1B polymorphisms, and high IL-1beta production may be associated with the grade of gastric atrophy in the corpus mucosa in patients with H. pylori infection.
BACKGROUND: Interkeukin-1 (IL-1) gene cluster polymorphisms that are thought to enhance the production of IL-1beta are associated with an increased risk of gastric cancer. To determine the role of host genetic factors in Helicobacter pylori infection, we examined the relationship between gastric mucosal IL-1beta levels and IL-1B polymorphisms in patients with H. pyloriinfection. METHODS: Biopsy tissues obtained from 99 patients were homogenized and gastric mucosal IL-1beta levels were measured by enzyme-linked immunosorbent assay (ELISA). Single-base polymorphisms at positions -511 and -31 in IL-1B were analyzed. RESULTS: The IL-1beta level in the antrum was significantly higher in genotype IL-1B-511C/C than in H. pylori-negative patients (P < 0.05). The IL-1B polymorphism did not influence the degree of gastric neutrophil and mononuclear cell infiltration, or gastric atrophy. IL-1beta levels in the corpus, but not those in the antrum, correlated to the severity of gastric atrophy. CONCLUSIONS: These findings indicate that IL-1B polymorphisms enhance IL-1beta production in the antrum; however, other factors might regulate the production of IL-1beta in the corpus of the stomach, regardless of IL-1B polymorphisms, and high IL-1beta production may be associated with the grade of gastric atrophy in the corpus mucosa in patients with H. pyloriinfection.
Authors: J C Machado; P Pharoah; S Sousa; R Carvalho; C Oliveira; C Figueiredo; A Amorim; R Seruca; C Caldas; F Carneiro; M Sobrinho-Simões Journal: Gastroenterology Date: 2001-10 Impact factor: 22.682
Authors: Sebastian Gehmert; Billie Velapatiño; Phabiola Herrera; Jaqueline Balqui; Livia Santivañez; Jaime Cok; Gloria Vargas; Juan Combe; Douglas J Passaro; Sijin Wen; Frank Meyer; Douglas E Berg; Robert H Gilman Journal: Am J Trop Med Hyg Date: 2009-11 Impact factor: 2.345
Authors: Camila A Figueiredo; Cintia Rodrigues Marques; Ryan dos Santos Costa; Hugo Bernardino F da Silva; Neuza M Alcantara-Neves Journal: World J Gastroenterol Date: 2014-05-14 Impact factor: 5.742