OBJECTIVE: Helicobacter pylori ( H.pylori ) infection is usually acquired in early childhood. Invasive techniques used for diagnosis of H.pylori infection require endoscopic examination which is expensive and inconvenient and may cause complications. The aim of this study was to evaluate the performance of a new noninvasive diagnostic method, stool antigen test for H.pylori in untreated children with recurrent abdominal pain. METHODS: Eighty children (35 female, 45 male) who have undergone upper gastrointestinal endoscopy due to recurrent abdominal pain were included in the study. The H.pylori stool antigen test (HpSA) is based on a sandwich enzyme immunoassay with antigen detection. HpSA sensitivity, specificity, and positive and negative predictive values were determined with reference to the results of both histology and rapid urease test as a gold standard ( H. pylori status). RESULTS: While 49 of the 80 children (61%) tested were positive for H.pylori according to the results of both histology and rapid urease test, 28 children had negative H.pylori status. Among those 49 children, 48 were found to be positive by HpSA. Of 28 patients with negative H.pylori status, 28 were H.pylori -negative also in the stool test. The sensitivity, specificity, and positive and negative predictive values of HpSA were found to be 98%, 100%, 100%, and 96.5%, respectively. CONCLUSION: These findings have demonstrated that HpSA as a relatively simple, inexpensive and time saving noninvasive test is a reliable method for detection of H.pylori infections in children.
OBJECTIVE:Helicobacter pylori ( H.pylori ) infection is usually acquired in early childhood. Invasive techniques used for diagnosis of H.pylori infection require endoscopic examination which is expensive and inconvenient and may cause complications. The aim of this study was to evaluate the performance of a new noninvasive diagnostic method, stool antigen test for H.pylori in untreated children with recurrent abdominal pain. METHODS: Eighty children (35 female, 45 male) who have undergone upper gastrointestinal endoscopy due to recurrent abdominal pain were included in the study. The H.pylori stool antigen test (HpSA) is based on a sandwich enzyme immunoassay with antigen detection. HpSA sensitivity, specificity, and positive and negative predictive values were determined with reference to the results of both histology and rapid urease test as a gold standard ( H. pylori status). RESULTS: While 49 of the 80 children (61%) tested were positive for H.pylori according to the results of both histology and rapid urease test, 28 children had negative H.pylori status. Among those 49 children, 48 were found to be positive by HpSA. Of 28 patients with negative H.pylori status, 28 were H.pylori -negative also in the stool test. The sensitivity, specificity, and positive and negative predictive values of HpSA were found to be 98%, 100%, 100%, and 96.5%, respectively. CONCLUSION: These findings have demonstrated that HpSA as a relatively simple, inexpensive and time saving noninvasive test is a reliable method for detection of H.pylori infections in children.
Authors: D Vaira; P Malfertheiner; F Mégraud; A T Axon; M Deltenre; A M Hirschl; G Gasbarrini; C O'Morain; J M Garcia; M Quina; G N Tytgat Journal: Lancet Date: 1999-07-03 Impact factor: 79.321
Authors: M O Husson; C Rolland; F Gottrand; D Guimber; N Kalach; C Spyckerelle; C Lenaerts; P S Ganga-Zandzou Journal: Eur J Clin Microbiol Infect Dis Date: 2000-10 Impact factor: 3.267
Authors: Pum Soo Kim; Jong Wook Lee; Soo Hwan Pai; Young Bae Kim; Jin Kyoung Cho; Jin Woo Lee; Seok Jeong; Don Haeng Lee; Hyung Gil Kim; Kye Sook Kwon; Hyeon Geun Cho; Yong Woon Shin; Young Soo Kim Journal: Yonsei Med J Date: 2002-02 Impact factor: 2.759
Authors: N L Arents; A A van Zwet; J C Thijs; A de Jong; M O Pool; J H Kleibeuker Journal: Eur J Gastroenterol Hepatol Date: 2001-04 Impact factor: 2.566
Authors: N Konstantopoulos; H Rüssmann; C Tasch; T Sauerwald; H Demmelmair; I Autenrieth; S Koletzko Journal: Am J Gastroenterol Date: 2001-03 Impact factor: 10.864
Authors: Y Vandenplas; U Blecker; T Devreker; E Keppens; J Nijs; S Cadranel; M Pipeleers-Marichal; A Goossens; S Lauwers Journal: Pediatrics Date: 1992-10 Impact factor: 7.124