OBJECTIVE: The noninvasive (13)C-urea breath test (UBT) is a reliable diagnostic method for detection of Helicobacter pylori infection in children, and it avoids invasive gastrointestinal endoscopy. In this study, we compared a noninvasive, newly developed fecal H pylori antigen test with the UBT. METHODOLOGY: One hundred sixty-two children (76 girls and 86 boys) were tested for H pylori infection using the UBT and a new antigen test in stool samples. The H pylori stool test is based on a sandwich enzyme immunoassay with antigen detection. RESULTS: Twenty-four of the children (14.8%) with dyspepsia tested positive for H pylori according to the breath test results. In 22 of the 24 patients, H pylori antigen could be detected in the stool (sensitivity: 91.6%). Of 138 patients with negative UBT results, 136 were H pylori-negative in the stool test (specificity: 98.6%). CONCLUSIONS: The new, noninvasive, low-cost H pylori antigen test in stool can replace the UBT for detection of H pylori infection in children with comparable reliability and accuracy.
OBJECTIVE: The noninvasive (13)C-urea breath test (UBT) is a reliable diagnostic method for detection of Helicobacter pyloriinfection in children, and it avoids invasive gastrointestinal endoscopy. In this study, we compared a noninvasive, newly developed fecal H pylori antigen test with the UBT. METHODOLOGY: One hundred sixty-two children (76 girls and 86 boys) were tested for H pylori infection using the UBT and a new antigen test in stool samples. The H pylori stool test is based on a sandwich enzyme immunoassay with antigen detection. RESULTS: Twenty-four of the children (14.8%) with dyspepsia tested positive for H pylori according to the breath test results. In 22 of the 24 patients, H pylori antigen could be detected in the stool (sensitivity: 91.6%). Of 138 patients with negative UBT results, 136 were H pylori-negative in the stool test (specificity: 98.6%). CONCLUSIONS: The new, noninvasive, low-cost H pylori antigen test in stool can replace the UBT for detection of H pylori infection in children with comparable reliability and accuracy.
Authors: Luciana de Carvalho Costa Cardinali; Gifone Aguiar Rocha; Andreia Maria Camargos Rocha; Sílvia Beleza de Moura; Taciana de Figueiredo Soares; Ana Maria Braz Esteves; Ana Margarida Miguel Ferreira Nogueira; Mônica Maria Demas Alvares Cabral; Anfrisina Sales Teles de Carvalho; Paulo Bitencourt; Alexandre Ferreira; Dulciene Maria Magalhães Queiroz Journal: J Clin Microbiol Date: 2003-07 Impact factor: 5.948