BACKGROUND: Fibulin-5 was recently found as a secreted extracellular matrix protein that functions as a scaffold for elastic fibres. However, the distribution of fibulin-5 in human skin and its changes during the ageing process are not known. OBJECTIVES: To explore the involvement of fibulin-5 in skin ageing, the age-dependent changes in fibulin-5 localization in human skin were examined compared with those of other elastic fibre components including elastin, fibrillin-1 and fibulin-2. Methods The distribution of elastin, fibrillin-1, fibrillin-2, fibulin-2 and fibulin-5 was investigated by means of immunohistochemistry using their specific antibodies. Skin samples were recovered from 12 healthy subjects undergoing plastic surgery. Ultraviolet (UV) B-irradiated or control nonirradiated buttock skin samples were obtained from two healthy volunteers at 2 days after the irradiation at 2 minimal erythemal doses. RESULTS: In the reticular dermis of young sun-protected skin from the upper arm, fibulin-5 colocalized with the other elastic fibre components, while in the papillary dermis fibulin-5 showed candelabra-like structures perpendicular to the epidermis with an unstained area just beneath the epidermis, which was similar to that of elastin but not fibrillin-1. Fibulin-5 in the reticular dermis decreased and disappeared with age even in sun-protected skin from the thigh, abdomen and upper arm. In sun-exposed skin, fibulin-5 was extremely reduced in the dermis of cheek skin even from a 20-year-old man. UVB irradiation reduced fibulin-5, fibulin-2 and elastin markedly, moderately and weakly, respectively, compared with levels in control nontreated skin. Interestingly, the deposition of fibulin-5 was increased in solar elastosis, like that of other elastic fibre components. CONCLUSIONS: These results suggest that fibulin-5 is a good marker of skin ageing and that the earlier loss of fibulin-5 may involve age-dependent changes in other elastic fibre components.
BACKGROUND:Fibulin-5 was recently found as a secreted extracellular matrix protein that functions as a scaffold for elastic fibres. However, the distribution of fibulin-5 in human skin and its changes during the ageing process are not known. OBJECTIVES: To explore the involvement of fibulin-5 in skin ageing, the age-dependent changes in fibulin-5 localization in human skin were examined compared with those of other elastic fibre components including elastin, fibrillin-1 and fibulin-2. Methods The distribution of elastin, fibrillin-1, fibrillin-2, fibulin-2 and fibulin-5 was investigated by means of immunohistochemistry using their specific antibodies. Skin samples were recovered from 12 healthy subjects undergoing plastic surgery. Ultraviolet (UV) B-irradiated or control nonirradiated buttock skin samples were obtained from two healthy volunteers at 2 days after the irradiation at 2 minimal erythemal doses. RESULTS: In the reticular dermis of young sun-protected skin from the upper arm, fibulin-5 colocalized with the other elastic fibre components, while in the papillary dermis fibulin-5 showed candelabra-like structures perpendicular to the epidermis with an unstained area just beneath the epidermis, which was similar to that of elastin but not fibrillin-1. Fibulin-5 in the reticular dermis decreased and disappeared with age even in sun-protected skin from the thigh, abdomen and upper arm. In sun-exposed skin, fibulin-5 was extremely reduced in the dermis of cheek skin even from a 20-year-old man. UVB irradiation reduced fibulin-5, fibulin-2 and elastin markedly, moderately and weakly, respectively, compared with levels in control nontreated skin. Interestingly, the deposition of fibulin-5 was increased in solar elastosis, like that of other elastic fibre components. CONCLUSIONS: These results suggest that fibulin-5 is a good marker of skin ageing and that the earlier loss of fibulin-5 may involve age-dependent changes in other elastic fibre components.
Authors: Matiss Ozols; Alexander Eckersley; Christopher I Platt; Callum Stewart-McGuinness; Sarah A Hibbert; Jerico Revote; Fuyi Li; Christopher E M Griffiths; Rachel E B Watson; Jiangning Song; Mike Bell; Michael J Sherratt Journal: Int J Mol Sci Date: 2021-03-17 Impact factor: 5.923
Authors: Matiss Ozols; Alexander Eckersley; Kieran T Mellody; Venkatesh Mallikarjun; Stacey Warwood; Ronan O'Cualain; David Knight; Rachel E B Watson; Christopher E M Griffiths; Joe Swift; Michael J Sherratt Journal: Aging Cell Date: 2021-04-08 Impact factor: 9.304
Authors: Sarah A Thurstan; Neil K Gibbs; Abigail K Langton; Christopher Em Griffiths; Rachel Eb Watson; Michael J Sherratt Journal: Chem Cent J Date: 2012-04-25 Impact factor: 4.215
Authors: Michaela Auer-Grumbach; Martin Weger; Regina Fink-Puches; Lea Papić; Eleonore Fröhlich; Piet Auer-Grumbach; Laila El Shabrawi-Caelen; Maria Schabhüttl; Christian Windpassinger; Jan Senderek; Herbert Budka; Slave Trajanoski; Andreas R Janecke; Anton Haas; Dieter Metze; Thomas R Pieber; Christian Guelly Journal: Brain Date: 2011-05-15 Impact factor: 13.501
Authors: Sarah A Hibbert; Rachel E B Watson; Neil K Gibbs; Patrick Costello; Clair Baldock; Anthony S Weiss; Christopher E M Griffiths; Michael J Sherratt Journal: Redox Biol Date: 2015-04-11 Impact factor: 11.799