Comparison of Buffalo green monkey kidney cells and McCoy cells for the isolation of Chlamydia trachomatis in shell vial centrifugation culture.

A total of 745 clinical specimens from patients attending hospitals and clinics in an area with a low prevalence of Chlamydia trachomatis were inoculated in parallel into shell vial cultures containing Buffalo green monkey kidney (BGM) and McCoy cells. The shell vial cultures were prepared in-house and were less than 5 days after seeding when inoculated. A total of 38 specimens (5%) were positive for C. trachomatis. In 36 of the cases, C. trachomatis was detected in both the BGM and McCoy vials. In two cases, C. trachomatis was detected in only the BGM vial. The BGM cells were more resistant to cytotoxicity and seemed to show more and larger inclusions than the McCoy cells. Furthermore, because the BGM cells displayed contact inhibition without the rounding and piling of cells that was encountered with the McCoy cells, they could be stored, ready to use, with an optical monolayer of cells, at 35 degrees C for at least 10 days.