Literature DB >> 16118008

Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli.

Ziqiang Guan1, Steven D Breazeale, Christian R H Raetz.   

Abstract

Undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc (lipid II) is extracted from Escherichia coli cells by utilizing its unusual pH-dependent solubility property in a Bligh-Dyer system, and identified by electrospray ionization mass spectrometry in conjunction with a novel 15N mass shift analysis. The described approach will facilitate the structural characterization of lipid II variants from diverse bacteria, including antibiotic-resistant mutants, as well as the numerous minor uncharacterized lipids present in all biological systems.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 16118008     DOI： 10.1016/j.ab.2005.07.002

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  21 in total

1.  A Burkholderia cenocepacia MurJ (MviN) homolog is essential for cell wall peptidoglycan synthesis and bacterial viability.

Authors:  Yasmine Fathy Mohamed; Miguel A Valvano
Journal:  Glycobiology       Date:  2014-03-31       Impact factor: 4.313

2.  Involvement of an essential gene, mviN, in murein synthesis in Escherichia coli.

Authors:  Azusa Inoue; Yoshimitsu Murata; Hiroshi Takahashi; Naoko Tsuji; Shingo Fujisaki; Jun-ichi Kato
Journal:  J Bacteriol       Date:  2008-08-15       Impact factor: 3.490

3.  Investigation of the conserved reentrant membrane helix in the monotopic phosphoglycosyl transferase superfamily supports key molecular interactions with polyprenol phosphate substrates.

Authors:  Sonya Entova; Ziqiang Guan; Barbara Imperiali
Journal:  Arch Biochem Biophys       Date:  2019-09-26       Impact factor: 4.013

4.  Identification of a chloroform-soluble membrane miniprotein in Escherichia coli and its homolog in Salmonella typhimurium.

Authors:  Ziqiang Guan; Xiaoyuan Wang; Christian R H Raetz
Journal:  Anal Biochem       Date:  2010-11-02       Impact factor: 3.365

Review 5.  Liquid chromatography/tandem mass spectrometry of dolichols and polyprenols, lipid sugar carriers across evolution.

Authors:  Ziqiang Guan; Jerry Eichler
Journal:  Biochim Biophys Acta       Date:  2011-05-04

6.  Non-enzymatically derived minor lipids found in Escherichia coli lipid extracts.

Authors:  Teresa A Garrett; Christian R H Raetz; Jennifer D Son; Travis D Richardson; Craig Bartling; Ziqiang Guan
Journal:  Biochim Biophys Acta       Date:  2011-08-24

7.  A novel epimerase that converts GlcNAc-P-P-undecaprenol to GalNAc-P-P-undecaprenol in Escherichia coli O157.

Authors:  Jeffrey S Rush; Cristina Alaimo; Riccardo Robbiani; Michael Wacker; Charles J Waechter
Journal:  J Biol Chem       Date:  2009-11-18       Impact factor: 5.157

8.  Identification of phosphatidylserylglutamate: a novel minor lipid in Escherichia coli.

Authors:  Teresa A Garrett; Christian R H Raetz; Travis Richardson; Reza Kordestani; Jennifer D Son; Rebecca L Rose
Journal:  J Lipid Res       Date:  2008-12-18       Impact factor: 5.922

9.  Identification of N-acylphosphatidylserine molecules in eukaryotic cells.

Authors:  Ziqiang Guan; Shengrong Li; Dale C Smith; Walter A Shaw; Christian R H Raetz
Journal:  Biochemistry       Date:  2007-11-22       Impact factor: 3.162

10.  Dodecaprenyl phosphate-galacturonic acid as a donor substrate for lipopolysaccharide core glycosylation in Rhizobium leguminosarum.

Authors:  Suparna Kanjilal-Kolar; Christian R H Raetz
Journal:  J Biol Chem       Date:  2006-02-23       Impact factor: 5.157
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.