OBJECTIVE: The influence of progressively high concentrations of platelet-rich plasma (PRP) on human osteoblast hFOB1.19 proliferation was evaluated. MATERIAL AND METHODS: The PRP was obtained from a human source. Two experiments were conducted. In the first one, PRP was diluted to 50%, 25%, 12.5% and 6.125% (v/v) with culture medium (Modified Eagle's Medium (MEM) : Ham's F12 Medium (HAM-F12%) and 1% antibiotics-antimicotic) supplemented with 10% of fetal bovine serum (FBS). In the second experiment, all conditions were identical except for the absence of FBS in the culture medium. RESULTS: The results of the osteoblast proliferation test were higher when stimulated by the 50% PRP dilution, with or without FBS. A further study is suggested to determine if concentrations above 50% could cause higher rates of osteoblast proliferation. In this study, the results were not statistically different (P<0.05) with 12.5% and 6.125% PRP dilutions. Additionally, it was shown that FBS is not necessary for PRP-mediated induction of osteoblast proliferation. CONCLUSION: This study concluded that PRP promotes osteoblast proliferation and suggested its clinical application to bone graft procedures in implant dentistry.
OBJECTIVE: The influence of progressively high concentrations of platelet-rich plasma (PRP) on human osteoblast hFOB1.19 proliferation was evaluated. MATERIAL AND METHODS: The PRP was obtained from a human source. Two experiments were conducted. In the first one, PRP was diluted to 50%, 25%, 12.5% and 6.125% (v/v) with culture medium (Modified Eagle's Medium (MEM) : Ham's F12 Medium (HAM-F12%) and 1% antibiotics-antimicotic) supplemented with 10% of fetal bovine serum (FBS). In the second experiment, all conditions were identical except for the absence of FBS in the culture medium. RESULTS: The results of the osteoblast proliferation test were higher when stimulated by the 50% PRP dilution, with or without FBS. A further study is suggested to determine if concentrations above 50% could cause higher rates of osteoblast proliferation. In this study, the results were not statistically different (P<0.05) with 12.5% and 6.125% PRP dilutions. Additionally, it was shown that FBS is not necessary for PRP-mediated induction of osteoblast proliferation. CONCLUSION: This study concluded that PRP promotes osteoblast proliferation and suggested its clinical application to bone graft procedures in implant dentistry.
Authors: Celio J da Costa Fernandes; Marcel Rodrigues Ferreira; Fábio J B Bezerra; Willian F Zambuzzi Journal: J Mater Sci Mater Med Date: 2018-03-26 Impact factor: 3.896
Authors: Marcos Andrade de Oliva; William Marcatti Amarú Maximiano; Larissa Moreira Spínola de Castro; Paulo Eliandro da Silva; Roger Rodrigo Fernandes; Pietro Ciancaglini; Márcio Mateus Beloti; Antonio Nanci; Adalberto Luiz Rosa; Paulo Tambasco de Oliveira Journal: J Histochem Cytochem Date: 2008-11-24 Impact factor: 2.479
Authors: Mahmoud A Saeed; Mohamed Abd El-Rahman; Mohamed E Helal; Ahmed R Zaher; Mohammed E Grawish Journal: Int J Stem Cells Date: 2017-05-30 Impact factor: 2.500