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Literature DB >> 16117513

A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins.

Yingqi Xu¹, Zhi Lin, Chien Ho, Daiwen Yang.

Abstract

A general strategy is proposed to assign aliphatic side-chain resonances of large 13C,15N-labeled proteins without deuteration, using 4D 13C,15N-edited NOESY and MQ-(H)CCH-TOCSY experiments on the basis of prior assignments of backbone and 13Cbeta resonances. The strategy has been tested on a 214 residue protein (DdCAD-1) and applied to a chain-selectively 13C,15N-labeled hemoglobin (65 kDa). About 96 and 80% aliphatic side-chain spins in DdCAD-1 and hemoglobin have been assigned, respectively. The strategy proposed here will be very useful for the structure determination and dynamics characterization of large proteins by NMR.

Entities: Chemical

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Year: 2005 PMID： 16117513 DOI： 10.1021/ja053539b

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

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Cited

8 in total

1. Observation of intermolecular interactions in large protein complexes by 2D-double difference nuclear Overhauser enhancement spectroscopy: application to the 44 kDa interferon-receptor complex.

Authors: Ilona Nudelman; Sabine R Akabayov; Tali Scherf; Jacob Anglister
Journal: J Am Chem Soc Date: 2011-08-23 Impact factor: 15.419

2. Methyl-detected 'out-and-back' NMR experiments for simultaneous assignments of Alabeta and Ilegamma2 methyl groups in large proteins.

Authors: Devon Sheppard; Chenyun Guo; Vitali Tugarinov
Journal: J Biomol NMR Date: 2009-03-10 Impact factor: 2.835

3. 4D Non-uniformly sampled C,C-NOESY experiment for sequential assignment of 13C, 15N-labeled RNAs.

Authors: Jan Stanek; Peter Podbevšek; Wiktor Koźmiński; Janez Plavec; Mirko Cevec
Journal: J Biomol NMR Date: 2013-08-21 Impact factor: 2.835

4. Structural basis for the Golgi association by the pleckstrin homology domain of the ceramide trafficking protein (CERT).

Authors: Toshihiko Sugiki; Koh Takeuchi; Toshiyuki Yamaji; Toshiaki Takano; Yuji Tokunaga; Keigo Kumagai; Kentaro Hanada; Hideo Takahashi; Ichio Shimada
Journal: J Biol Chem Date: 2012-08-06 Impact factor: 5.157

5. Intermolecular interactions in a 44 kDa interferon-receptor complex detected by asymmetric reverse-protonation and two-dimensional NOESY.

Authors: Ilona Nudelman; Sabine R Akabayov; Einat Schnur; Zohar Biron; Rina Levy; Yingqi Xu; Daiwen Yang; Jacob Anglister
Journal: Biochemistry Date: 2010-06-29 Impact factor: 3.162

A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins.

1. Observation of intermolecular interactions in large protein complexes by 2D-double difference nuclear Overhauser enhancement spectroscopy: application to the 44 kDa interferon-receptor complex.

2. Methyl-detected 'out-and-back' NMR experiments for simultaneous assignments of Alabeta and Ilegamma2 methyl groups in large proteins.

3. 4D Non-uniformly sampled C,C-NOESY experiment for sequential assignment of 13C, 15N-labeled RNAs.

4. Structural basis for the Golgi association by the pleckstrin homology domain of the ceramide trafficking protein (CERT).

5. Intermolecular interactions in a 44 kDa interferon-receptor complex detected by asymmetric reverse-protonation and two-dimensional NOESY.

6. An extended combinatorial 15N, 13Cα, and 13C' labeling approach to protein backbone resonance assignment.

7. CcpNmr AnalysisAssign: a flexible platform for integrated NMR analysis.

8. Structure of the C-terminal domain of TRADD reveals a novel fold in the death domain superfamily.