AIMS: This study examined the tolerance of various species of the genus Bifidobacterium to heat and oxygen and evaluated the survival of selected strains following spray drying and during storage. METHODS AND RESULTS: Nine Bifidobacterium species were considered to be relatively tolerant to both heat and oxygen and mostly segregated into two clusters within the 16S rDNA phylogenetic tree. Four species were tolerant to oxygen and 12 species were considered sensitive to oxygen and heat. Using a skimmed milk-based carrier good survival following spray drying and storage at 4 degrees C correlated with tolerance to heat and oxygen. Viability was inversely related to storage temperature and at 15 degrees C and 25 degrees C, a significant decline was observed for all species. The inclusion of gum acacia had no significant affect on survival or viability. However, using a fluidized-bed spray dryer viability was greatly improved. CONCLUSIONS: A group of closely related species tolerant to heat and oxygen had high survival following spray drying and maintained viability during prolonged storage at 4 degrees C. SIGNIFICANCE AND IMPACT OF THE STUDY: Spray drying is a suitable method for the production of skimmed milk powder enriched with high numbers of viable bifidobacteria.
AIMS: This study examined the tolerance of various species of the genus Bifidobacterium to heat and oxygen and evaluated the survival of selected strains following spray drying and during storage. METHODS AND RESULTS: Nine Bifidobacterium species were considered to be relatively tolerant to both heat and oxygen and mostly segregated into two clusters within the 16S rDNA phylogenetic tree. Four species were tolerant to oxygen and 12 species were considered sensitive to oxygen and heat. Using a skimmed milk-based carrier good survival following spray drying and storage at 4 degrees C correlated with tolerance to heat and oxygen. Viability was inversely related to storage temperature and at 15 degrees C and 25 degrees C, a significant decline was observed for all species. The inclusion of gum acacia had no significant affect on survival or viability. However, using a fluidized-bed spray dryer viability was greatly improved. CONCLUSIONS: A group of closely related species tolerant to heat and oxygen had high survival following spray drying and maintained viability during prolonged storage at 4 degrees C. SIGNIFICANCE AND IMPACT OF THE STUDY: Spray drying is a suitable method for the production of skimmed milk powder enriched with high numbers of viable bifidobacteria.
Authors: Joseph R Loquasto; Rodolphe Barrangou; Edward G Dudley; Buffy Stahl; Chun Chen; Robert F Roberts Journal: Appl Environ Microbiol Date: 2013-08-30 Impact factor: 4.792
Authors: Annereinou R Dijkstra; Meily C Setyawati; Jumamurat R Bayjanov; Wynand Alkema; Sacha A F T van Hijum; Peter A Bron; Jeroen Hugenholtz Journal: Appl Environ Microbiol Date: 2013-11-08 Impact factor: 4.792
Authors: Lorena Ruiz; Patricia Ruas-Madiedo; Miguel Gueimonde; Clara G de Los Reyes-Gavilán; Abelardo Margolles; Borja Sánchez Journal: Genes Nutr Date: 2011-01-13 Impact factor: 5.523
Authors: Gabriele Andrea Lugli; Walter Mancino; Christian Milani; Sabrina Duranti; Leonardo Mancabelli; Stefania Napoli; Marta Mangifesta; Alice Viappiani; Rosaria Anzalone; Giulia Longhi; Douwe van Sinderen; Marco Ventura; Francesca Turroni Journal: Appl Environ Microbiol Date: 2019-03-22 Impact factor: 4.792