PURPOSE: To evaluate whether static high magnetic fields (HMFs), in the range of 10-17 T, affect the cytoskeleton and cell organization in different types of mammalian cells, including fibroblasts, epithelial cells, and differentiating neurons. MATERIALS AND METHODS: Cells were exposed to HMF for 30 or 60 minutes and subsequently assessed for viability. Cytoskeleton arrays and focal adhesions were visualized using immunofluorescence microscopy. RESULTS: Cell exposure to HMF over 10 T in the case of cycling cells, and over 15 T in the case of neurons, affected cell viability, apparently because of cell detachment from culture dishes. In the remaining adherent cells, the organization of actin assemblies was perturbed, and both cell adhesion and spreading were impaired. Moreover, in the case of neurons, exposure to HMF induced growth cone retraction and delayed cell differentiation. CONCLUSION: Cell exposure to HMF (over 10T and 15 T in the case of cycling cells and neurons, respectively) affects the cell cytoskeleton, with deleterious effects on cell viability, organization, and differentiation. Further studies are needed to determine whether such perturbations, as observed here in cultured cells, have consequences in whole animals. (c) 2005 Wiley-Liss, Inc.
PURPOSE: To evaluate whether static high magnetic fields (HMFs), in the range of 10-17 T, affect the cytoskeleton and cell organization in different types of mammalian cells, including fibroblasts, epithelial cells, and differentiating neurons. MATERIALS AND METHODS: Cells were exposed to HMF for 30 or 60 minutes and subsequently assessed for viability. Cytoskeleton arrays and focal adhesions were visualized using immunofluorescence microscopy. RESULTS: Cell exposure to HMF over 10 T in the case of cycling cells, and over 15 T in the case of neurons, affected cell viability, apparently because of cell detachment from culture dishes. In the remaining adherent cells, the organization of actin assemblies was perturbed, and both cell adhesion and spreading were impaired. Moreover, in the case of neurons, exposure to HMF induced growth cone retraction and delayed cell differentiation. CONCLUSION: Cell exposure to HMF (over 10T and 15 T in the case of cycling cells and neurons, respectively) affects the cell cytoskeleton, with deleterious effects on cell viability, organization, and differentiation. Further studies are needed to determine whether such perturbations, as observed here in cultured cells, have consequences in whole animals. (c) 2005 Wiley-Liss, Inc.
Authors: Thomas F Budinger; Mark D Bird; Lucio Frydman; Joanna R Long; Thomas H Mareci; William D Rooney; Bruce Rosen; John F Schenck; Victor D Schepkin; A Dean Sherry; Daniel K Sodickson; Charles S Springer; Keith R Thulborn; Kamil Uğurbil; Lawrence L Wald Journal: MAGMA Date: 2016-05-18 Impact factor: 2.310
Authors: Valentina Hartwig; Giulio Giovannetti; Nicola Vanello; Massimo Lombardi; Luigi Landini; Silvana Simi Journal: Int J Environ Res Public Health Date: 2009-06-10 Impact factor: 3.390
Authors: Caroline Crespo da Costa; Léo Anderson Meira Martins; André Peres Koth; Jéssica Marques Obelar Ramos; Fátima Theresinha Costa Rodrigues Guma; Cleverson Moraes de Oliveira; Nathália Stark Pedra; Geferson Fischer; Eduarda Santa Helena; Carolina Rosa Gioda; Paulo Roberto Stefani Sanches; Antonio Sergio Varela Junior; Mayara Sandrielly Pereira Soares; Rosélia Maria Spanevello; Giovana Duzzo Gamaro; Izabel Cristina Custódio de Souza Journal: Cell Biochem Biophys Date: 2021-06-27 Impact factor: 2.194