Literature DB >> 16092832

N-acyl derivatives of arginine and tryptophan isolated from environmental DNA expressed in Escherichia coli.

Sean F Brady1, Jon Clardy.   

Abstract

Heterologous expression of microbial DNA extracted directly from environmental samples (environmental DNA, eDNA) in easily cultured hosts can provide access to natural products produced by previously uncultured bacteria. This report describes the characterization of antibacterially active long-chain N-acyl derivatives of tryptophan and arginine that are produced by eDNA clones hosted in Escherichia coli. The sequencing and subcloning of the proposed N-acyl amino acid synthases (NASs) for each family of natural products are also described. [reaction: see text]

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Year:  2005        PMID： 16092832     DOI： 10.1021/ol0509585

Source DB:  PubMed          Journal:  Org Lett        ISSN： 1523-7052            Impact factor:   6.005


  13 in total

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2.  Long-chain N-acyl amino acid synthases are linked to the putative PEP-CTERM/exosortase protein-sorting system in Gram-negative bacteria.

Authors:  Jeffrey W Craig; Marisa A Cherry; Sean F Brady
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3.  Synthetic libraries of tyrosine-derived bacterial metabolites.

Authors:  Savvas N Georgiades; Jon Clardy
Journal:  Bioorg Med Chem Lett       Date:  2007-10-22       Impact factor: 2.823

4.  Expanding small-molecule functional metagenomics through parallel screening of broad-host-range cosmid environmental DNA libraries in diverse proteobacteria.

Authors:  Jeffrey W Craig; Fang-Yuan Chang; Jeffrey H Kim; Steven C Obiajulu; Sean F Brady
Journal:  Appl Environ Microbiol       Date:  2010-01-15       Impact factor: 4.792

5.  Functional metagenomic discovery of bacterial effectors in the human microbiome and isolation of commendamide, a GPCR G2A/132 agonist.

Authors:  Louis J Cohen; Hahk-Soo Kang; John Chu; Yun-Han Huang; Emma A Gordon; Boojala Vijay B Reddy; Melinda A Ternei; Jeffrey W Craig; Sean F Brady
Journal:  Proc Natl Acad Sci U S A       Date:  2015-08-17       Impact factor: 11.205

6.  Cloning large natural product gene clusters from the environment: piecing environmental DNA gene clusters back together with TAR.

Authors:  Jeffrey H Kim; Zhiyang Feng; John D Bauer; Dimitris Kallifidas; Paula Y Calle; Sean F Brady
Journal:  Biopolymers       Date:  2010-09       Impact factor: 2.505

7.  Cyclic AMP directly activates NasP, an N-acyl amino acid antibiotic biosynthetic enzyme cloned from an uncultured beta-proteobacterium.

Authors:  Jon Clardy; Sean F Brady
Journal:  J Bacteriol       Date:  2007-06-22       Impact factor: 3.490

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Journal:  J Nat Prod       Date:  2016-01-29       Impact factor: 4.050

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Journal:  Sci Rep       Date:  2016-06-08       Impact factor: 4.379

10.  The Bacteroidales produce an N-acylated derivative of glycine with both cholesterol-solubilising and hemolytic activity.

Authors:  Alli Lynch; Elaine Crowley; Eoghan Casey; Rafael Cano; Rachel Shanahan; Ger McGlacken; Julian R Marchesi; David J Clarke
Journal:  Sci Rep       Date:  2017-10-16       Impact factor: 4.379
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