C M Galhardi1, Y S Diniz, H G Rodrigues, L A Faine, R C Burneiko, B O Ribas, E L B Novelli. 1. Department of Chemistry and Biochemistry, Institute of Biological Sciences and Post-Graduation Course of Clinical, Cardiology, Faculty of Medicine, University Estadual Paulista, UNESP, Botucatu, São Paulo, Brazil.
Abstract
BACKGROUND: A nutrition experiment was utilized to investigate the effects of two levels of dietary copper (Cu) supplementation on lipid profile and antioxidant defenses in serum of rats. METHODS: Male Wistar rats (180-200 g; n = 10) were divided into three groups: control group (A), fed a basal diet with 6 microg Cu/g, and rats fed a basal diet with Cu (CuSO4) supplementation from aqueous solutions, for 4 weeks at the final concentrations of 2 mg Cu/rat (B) and 3 mg Cu/rat (C). RESULTS: No significant changes were observed in final body weight, body weight gain, food consumption, total serum protein and high-density lipoprotein. Cu supplementation reduced the triacylglycerol (TG), total cholesterol and low-density lipoprotein (LDL-C). The LDL-C/TG ratio and total antioxidant substances (TAS) were higher in (B) and (C) groups than in (A) group. There was a positive correlation between Cu supplementation and ceruloplasmin levels. The markers of oxidative stress, lipid hydroperoxide and lipoperoxide were decreased with Cu supplementation. No alterations were observed in superoxide dismutase, indicating saturation of Cu enzyme site. The glutathione peroxidase activities (GSH-Px) were increased in both Cu-supplemented groups. Considering that a copper-selenium interaction can affect mineral availability of both elements, the effects of Cu on TAS and GSH-Px activities were associated with increased selenium disposal. CONCLUSIONS: Dietary Cu supplementation had beneficial effects on lipid profile by improving endogenous antioxidant defenses and decreasing the oxidative stress in vivo. Copyright (c) 2005 S. Karger AG, Basel.
BACKGROUND: A nutrition experiment was utilized to investigate the effects of two levels of dietary copper (Cu) supplementation on lipid profile and antioxidant defenses in serum of rats. METHODS: Male Wistar rats (180-200 g; n = 10) were divided into three groups: control group (A), fed a basal diet with 6 microg Cu/g, and rats fed a basal diet with Cu (CuSO4) supplementation from aqueous solutions, for 4 weeks at the final concentrations of 2 mg Cu/rat (B) and 3 mg Cu/rat (C). RESULTS: No significant changes were observed in final body weight, body weight gain, food consumption, total serum protein and high-density lipoprotein. Cu supplementation reduced the triacylglycerol (TG), total cholesterol and low-density lipoprotein (LDL-C). The LDL-C/TG ratio and total antioxidant substances (TAS) were higher in (B) and (C) groups than in (A) group. There was a positive correlation between Cu supplementation and ceruloplasmin levels. The markers of oxidative stress, lipid hydroperoxide and lipoperoxide were decreased with Cu supplementation. No alterations were observed in superoxide dismutase, indicating saturation of Cu enzyme site. The glutathione peroxidase activities (GSH-Px) were increased in both Cu-supplemented groups. Considering that a copper-selenium interaction can affect mineral availability of both elements, the effects of Cu on TAS and GSH-Px activities were associated with increased selenium disposal. CONCLUSIONS: Dietary Cu supplementation had beneficial effects on lipid profile by improving endogenous antioxidant defenses and decreasing the oxidative stress in vivo. Copyright (c) 2005 S. Karger AG, Basel.
Authors: Jessica Seessle; Annina Gohdes; Daniel Nils Gotthardt; Jan Pfeiffenberger; Nicola Eckert; Wolfgang Stremmel; Ulrike Reuner; Karl Heinz Weiss Journal: Lipids Health Dis Date: 2011-05-19 Impact factor: 3.876
Authors: Marta Galvez-Fernandez; Francisco Sanchez-Saez; Arce Domingo-Relloso; Zulema Rodriguez-Hernandez; Sonia Tarazona; Vannina Gonzalez-Marrachelli; Maria Grau-Perez; Jose M Morales-Tatay; Nuria Amigo; Tamara Garcia-Barrera; Jose L Gomez-Ariza; F Javier Chaves; Ana Barbara Garcia-Garcia; Rebeca Melero; Maria Tellez-Plaza; Juan C Martin-Escudero; Josep Redon; Daniel Monleon Journal: Redox Biol Date: 2022-04-14 Impact factor: 10.787