BACKGROUND: In a previous community-based cohort study in Guinea-Bissau from 1996 to 1998, characterisation of rotavirus strains showed a high frequency of less common genotypes such as G8 and G9 and a high proportion of mixed infections. OBJECTIVES AND STUDY DESIGN: In the present study, we examined the prevalence of rotavirus genotypes among 81 hospitalised and 23 non-hospitalised Guinean children with rotavirus associated diarrhoea during the 2002 seasonal rotavirus outbreak. G- and P-types were determined in a two-step procedure using reverse transcription followed by a standard multiplex PCR. The multiplex PCR for G-types was furthermore supplemented with a single locus PCR including the MW8 primer for the G8-genotype. RESULTS: The dual infection G2/P[4]P[6] (24%) appeared to be the most frequent cause of rotavirus infections followed by G2P[4] (19%), G2P[6] (16%) and G8P[6] (13%). Overall 38% of the infections were mixed and 18% of the samples had the genotype G8. However, by subjecting all samples and not only the strains, which according to the standard multiplex PCR procedure were non-typeable, to a single locus G8-PCR, we found that the genotype G8 appeared in 62% of the infections, either as a single G-strain or in combination with other G-types, especially G2. Including these results, more than 63% of infections emerged as mixed. Neither genotype (including the presence of G8) nor the presence of mixed infections, seem to influence the severity of the rotavirus infection. CONCLUSION: We found a high frequency of mixed infections especially due to G8-genotypes, which might have implications for development of rotavirus vaccine candidates for use in Africa. Our results do not suggest that a single genotype is associated with severity, but the present study is based on a modest number of samples and results should be interpreted with caution.
BACKGROUND: In a previous community-based cohort study in Guinea-Bissau from 1996 to 1998, characterisation of rotavirus strains showed a high frequency of less common genotypes such as G8 and G9 and a high proportion of mixed infections. OBJECTIVES AND STUDY DESIGN: In the present study, we examined the prevalence of rotavirus genotypes among 81 hospitalised and 23 non-hospitalised Guinean children with rotavirus associated diarrhoea during the 2002 seasonal rotavirus outbreak. G- and P-types were determined in a two-step procedure using reverse transcription followed by a standard multiplex PCR. The multiplex PCR for G-types was furthermore supplemented with a single locus PCR including the MW8 primer for the G8-genotype. RESULTS: The dual infection G2/P[4]P[6] (24%) appeared to be the most frequent cause of rotavirus infections followed by G2P[4] (19%), G2P[6] (16%) and G8P[6] (13%). Overall 38% of the infections were mixed and 18% of the samples had the genotype G8. However, by subjecting all samples and not only the strains, which according to the standard multiplex PCR procedure were non-typeable, to a single locus G8-PCR, we found that the genotype G8 appeared in 62% of the infections, either as a single G-strain or in combination with other G-types, especially G2. Including these results, more than 63% of infections emerged as mixed. Neither genotype (including the presence of G8) nor the presence of mixed infections, seem to influence the severity of the rotavirus infection. CONCLUSION: We found a high frequency of mixed infections especially due to G8-genotypes, which might have implications for development of rotavirus vaccine candidates for use in Africa. Our results do not suggest that a single genotype is associated with severity, but the present study is based on a modest number of samples and results should be interpreted with caution.
Authors: Norma Santos; Shinjiro Honma; Maria do Carmo S T Timenetsky; Alexandre C Linhares; Hiroshi Ushijima; George E Armah; Jon R Gentsch; Yasutaka Hoshino Journal: J Clin Microbiol Date: 2007-12-05 Impact factor: 5.948
Authors: T Grassi; F Bagordo; A Cavallaro; M Guido; C Malaventura; G Gabutti; A De Donno Journal: Eur J Clin Microbiol Infect Dis Date: 2011-07-28 Impact factor: 3.267
Authors: Jie Liu; Kate Lurain; Shihab U Sobuz; Sharmin Begum; Happiness Kumburu; Jean Gratz; Gibson Kibiki; Denise Toney; Rashi Gautam; Michael D Bowen; William A Petri; Rashidul Haque; Eric R Houpt Journal: J Virol Methods Date: 2014-12-17 Impact factor: 2.014
Authors: E R L Freitas; C M A Soares; F S Fiaccadori; M Souza; J A Parente; P S S Costa; D D P Cardoso Journal: Eur J Clin Microbiol Infect Dis Date: 2008-06-03 Impact factor: 3.267