| Literature DB >> 16080266 |
Tetsuichi Wazawa1, Masahiro Ueda.
Abstract
Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of biomolecules at the single-molecule level both in vitro and in living cells. This technique has opened a new era in bioscience, so-called single-molecule nanobioscience. In this review, we will summarize the theoretical basis of TIRFM, explain the instruments of TIRFM for single-molecules imaging, and discuss some novel applications of TIRFM to single-molecule nanobioscience.Mesh:
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Year: 2005 PMID: 16080266 DOI: 10.1007/b102211
Source DB: PubMed Journal: Adv Biochem Eng Biotechnol ISSN: 0724-6145 Impact factor: 2.635