Literature DB >> 16074986

Simple bioseparations using self-cleaving elastin-like polypeptide tags.

Mahmoud Reza Banki1, Liang Feng, David W Wood.   

Abstract

We introduce a new method for the purification of recombinant proteins expressed in Escherichia coli using self-cleaving elastin-like polypeptide (ELP) fusion tags without the need for affinity chromatography or proteolytic tag removal. Using this method we obtained high purity, activity and reasonable yields for ten diverse target proteins.

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Year:  2005        PMID： 16074986     DOI： 10.1038/nmeth787

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  46 in total

1.  Purification of recombinant proteins from Escherichia coli at low expression levels by inverse transition cycling.

Authors:  Trine Christensen; Kimberly Trabbic-Carlson; Wenge Liu; Ashutosh Chilkoti
Journal:  Anal Biochem       Date:  2006-11-03       Impact factor: 3.365

2.  Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides.

Authors:  Dong Woo Lim; Kimberly Trabbic-Carlson; J Andrew Mackay; Ashutosh Chilkoti
Journal:  Biomacromolecules       Date:  2007-04-04       Impact factor: 6.988

3.  Peptide-based Biopolymers in Biomedicine and Biotechnology.

Authors:  Dominic Chow; Michelle L Nunalee; Dong Woo Lim; Andrew J Simnick; Ashutosh Chilkoti
Journal:  Mater Sci Eng R Rep       Date:  2008-01       Impact factor: 36.214

Review 4.  To fuse or not to fuse: what is your purpose?

Authors:  Mark R Bell; Mark J Engleka; Asim Malik; James E Strickler
Journal:  Protein Sci       Date:  2013-09-17       Impact factor: 6.725

5.  Electronic structure of neighboring extein residue modulates intein C-terminal cleavage activity.

Authors:  Philip T Shemella; Natalya I Topilina; Ikko Soga; Brian Pereira; Georges Belfort; Marlene Belfort; Saroj K Nayak
Journal:  Biophys J       Date:  2011-05-04       Impact factor: 4.033

6.  Monitoring the conformational changes of an intrinsically disordered peptide using a quartz crystal microbalance.

Authors:  Oren Shur; Jun Wu; Donald M Cropek; Scott Banta
Journal:  Protein Sci       Date:  2011-04-08       Impact factor: 6.725

7.  Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides.

Authors:  Joseph J Bellucci; Miriam Amiram; Jayanta Bhattacharyya; Dewey McCafferty; Ashutosh Chilkoti
Journal:  Angew Chem Int Ed Engl       Date:  2013-02-19       Impact factor: 15.336

8.  Allosteric actuation of inverse phase transition of a stimulus-responsive fusion polypeptide by ligand binding.

Authors:  Bumjoon Kim; Ashutosh Chilkoti
Journal:  J Am Chem Soc       Date:  2008-12-31       Impact factor: 15.419

9.  Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification.

Authors:  Shuang Zhang; Zhi Hui Wang; Guo Qiang Chen
Journal:  Microb Cell Fact       Date:  2010-05-10       Impact factor: 5.328

10.  Simplified, enhanced protein purification using an inducible, autoprocessing enzyme tag.

Authors:  Aimee Shen; Patrick J Lupardus; Montse Morell; Elizabeth L Ponder; A Masoud Sadaghiani; K Christopher Garcia; Matthew Bogyo
Journal:  PLoS One       Date:  2009-12-02       Impact factor: 3.240
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