| Literature DB >> 16061230 |
Yusuke Temmei1, Shinichi Uchida, Daisuke Hoshino, Nobuyuki Kanzawa, Michio Kuwahara, Sei Sasaki, Takahide Tsuchiya.
Abstract
cDNAs encoding aquaporins PIP1;1, PIP2;1, and TIP1;1 were isolated from Mimosa pudica (Mp) cDNA library. MpPIP1;1 exhibited no water channel activity; however, it facilitated the water channel activity of MpPIP2;1 in a phosphorylation-dependent manner. Mutagenesis analysis revealed that Ser-131 of MpPIP1;1 was phosphorylated by PKA and that cooperative regulation of the water channel activity of MpPIP2;1 was regulated by phosphorylation of Ser-131 of MpPIP1;1. Immunoprecipitation analysis revealed that MpPIP1;1 binds directly to MpPIP2;1 in a phosphorylation-independent manner, suggesting that phosphorylation of Ser-131 of MpPIP1;1 is involved in regulation of the structure of the channel complex with MpMIP2;1 and thereby affects water channel activity.Entities:
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Year: 2005 PMID： 16061230 DOI： 10.1016/j.febslet.2005.06.082
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124