Literature DB >> 16029966

Pseudouridine detection improvement by derivatization with methyl vinyl sulfone and capillary HPLC-mass spectrometry.

Gert Emmerechts1, Piet Herdewijn, Jef Rozenski.   

Abstract

A method is presented for improved detection of pseudouridine in nucleoside mixtures based on the specific derivatization with methyl vinyl sulfone followed by analysis by capillary HPLC-mass spectrometry. Reaction conditions were optimized in order to obtain the best yield and specificity. The method was successfully applied to different nucleoside mixtures.

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Year:  2005        PMID： 16029966     DOI： 10.1016/j.jchromb.2005.06.041

Source DB:  PubMed          Journal:  J Chromatogr B Analyt Technol Biomed Life Sci        ISSN： 1570-0232            Impact factor:   3.205


  10 in total

1.  Mass spectrometry-based quantification of pseudouridine in RNA.

Authors:  Balasubrahmanyam Addepalli; Patrick A Limbach
Journal:  J Am Soc Mass Spectrom       Date:  2011-05-03       Impact factor: 3.109

Review 2.  Expanding the nucleotide repertoire of the ribosome with post-transcriptional modifications.

Authors:  Christine S Chow; Tek N Lamichhane; Santosh K Mahto
Journal:  ACS Chem Biol       Date:  2007-09-21       Impact factor: 5.100

3.  Improving CMC-derivatization of pseudouridine in RNA for mass spectrometric detection.

Authors:  Anita Durairaj; Patrick A Limbach
Journal:  Anal Chim Acta       Date:  2008-02-17       Impact factor: 6.558

Review 4.  Mass spectrometry of the fifth nucleoside: a review of the identification of pseudouridine in nucleic acids.

Authors:  Anita Durairaj; Patrick A Limbach
Journal:  Anal Chim Acta       Date:  2008-06-26       Impact factor: 6.558

5.  Use of specific chemical reagents for detection of modified nucleotides in RNA.

Authors:  Isabelle Behm-Ansmant; Mark Helm; Yuri Motorin
Journal:  J Nucleic Acids       Date:  2011-04-13

Review 6.  The identification and characterization of non-coding and coding RNAs and their modified nucleosides by mass spectrometry.

Authors:  Kirk W Gaston; Patrick A Limbach
Journal:  RNA Biol       Date:  2014       Impact factor: 4.652

Review 7.  Detection of nucleic acid modifications by chemical reagents.

Authors:  Matthias Heiss; Stefanie Kellner
Journal:  RNA Biol       Date:  2016-11-30       Impact factor: 4.652

8.  Post-transcriptional modification mapping in the Clostridium acetobutylicum 16S rRNA by mass spectrometry and reverse transcriptase assays.

Authors:  Gert Emmerechts; Sofie Barbé; Piet Herdewijn; Jozef Anné; Jef Rozenski
Journal:  Nucleic Acids Res       Date:  2007-05-03       Impact factor: 16.971

9.  Structure-function relationship of substituted bromomethylcoumarins in nucleoside specificity of RNA alkylation.

Authors:  Stefanie Kellner; Laura Bettina Kollar; Antonia Ochel; Manjunath Ghate; Mark Helm
Journal:  PLoS One       Date:  2013-07-03       Impact factor: 3.240

10.  Pharmacokinetic Studies of Antisense Oligonucleotides Using MALDI-TOF Mass Spectrometry.

Authors:  Markus Herkt; Ariana Foinquinos; Sandor Batkai; Thomas Thum; Andreas Pich
Journal:  Front Pharmacol       Date:  2020-03-25       Impact factor: 5.810
  10 in total

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