Literature DB >> 16027900

Identification of broad specificity P450CAM variants by primary screening against indole as substrate.

Ayhan Celik1, Robert E Speight, Nicholas J Turner.   

Abstract

High-throughput screening of cytochrome P450CAM libraries, for their ability to oxidise indole to indigo and indirubin, has resulted in the identification of variants with activity towards the structurally unrelated substrate diphenylmethane.

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Year:  2005        PMID： 16027900     DOI： 10.1039/b506156c

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  7 in total

1.  Identification of amino acid residues involved in 4-chloroindole 3-hydroxylation by cytochrome P450 2A6 using screening of random libraries.

Authors:  Zhi-Gang Zhang; Yan Liu; F Peter Guengerich; Johannes H Matse; Jun Chen; Zhong-Liu Wu
Journal:  J Biotechnol       Date:  2008-10-15       Impact factor: 3.307

2.  Detoxification of Indole by an Indole-Induced Flavoprotein Oxygenase from Acinetobacter baumannii.

Authors:  Guang-Huey Lin; Hao-Ping Chen; Hung-Yu Shu
Journal:  PLoS One       Date:  2015-09-21       Impact factor: 3.240

3.  Evolution and enrichment of CYP5035 in Polyporales: functionality of an understudied P450 family.

Authors:  Nico D Fessner; David R Nelson; Anton Glieder
Journal:  Appl Microbiol Biotechnol       Date:  2021-08-30       Impact factor: 4.813

4.  Biosynthetic Functional Gene Analysis of Bis-Indole Metabolites from 25D7, a Clone Derived from a Deep-Sea Sediment Metagenomic Library.

Authors:  Xia Yan; Xi-Xiang Tang; Dan Qin; Zhi-Wei Yi; Mei-Juan Fang; Zhen Wu; Ying-Kun Qiu
Journal:  Mar Drugs       Date:  2016-06-01       Impact factor: 5.118

5.  Active site diversification of P450cam with indole generates catalysts for benzylic oxidation reactions.

Authors:  Paul P Kelly; Anja Eichler; Susanne Herter; David C Kranz; Nicholas J Turner; Sabine L Flitsch
Journal:  Beilstein J Org Chem       Date:  2015-09-22       Impact factor: 2.883

6.  Engineering of a chromogenic enzyme screening system based on an auxiliary indole-3-carboxylic acid monooxygenase.

Authors:  Vida Časaitė; Mikas Sadauskas; Justas Vaitekūnas; Renata Gasparavičiūtė; Rita Meškienė; Izabelė Skikaitė; Mantas Sakalauskas; Jevgenija Jakubovska; Daiva Tauraitė; Rolandas Meškys
Journal:  Microbiologyopen       Date:  2019-01-21       Impact factor: 3.139

7.  A 96-multiplex capillary electrophoresis screening platform for product based evolution of P450 BM3.

Authors:  Anna Gärtner; Anna Joëlle Ruff; Ulrich Schwaneberg
Journal:  Sci Rep       Date:  2019-10-29       Impact factor: 4.379
  7 in total

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