PURPOSE: To evaluate the toxicity of indocyanine green (ICG) in combination with light. METHODS: Human retinal pigment epithelial cells (ARPE-19) and rat neurosensory retinal cells (R28) were treated with four different concentrations of ICG in combination with light exposure. Cell viability, mitochondrial function, and DNA synthesis were measured. RESULTS: All concentrations of ICG with 10 min of light exposure caused a significant decrease in mitochondrial dehydrogenase activity in R28 and ARPE-19 cells. ICG without light exposure did not decrease mitochondrial dehydrogenase activity. In both cell lines, [(3)H]thymidine incorporation was increased when treated with ICG with or without light. R28 cells did not show any significant decrease in cell viability. CONCLUSIONS: The duration of light was a significant factor in ICG toxicity. ICG needs to be used with caution as it decreases the mitochondrial dehydrogenase activity and increases the DNA synthesis in retinal cells, markers for cell toxicity and dysfunction.
PURPOSE: To evaluate the toxicity of indocyanine green (ICG) in combination with light. METHODS:Human retinal pigment epithelial cells (ARPE-19) and rat neurosensory retinal cells (R28) were treated with four different concentrations of ICG in combination with light exposure. Cell viability, mitochondrial function, and DNA synthesis were measured. RESULTS: All concentrations of ICG with 10 min of light exposure caused a significant decrease in mitochondrial dehydrogenase activity in R28 and ARPE-19 cells. ICG without light exposure did not decrease mitochondrial dehydrogenase activity. In both cell lines, [(3)H]thymidine incorporation was increased when treated with ICG with or without light. R28 cells did not show any significant decrease in cell viability. CONCLUSIONS: The duration of light was a significant factor in ICGtoxicity. ICG needs to be used with caution as it decreases the mitochondrial dehydrogenase activity and increases the DNA synthesis in retinal cells, markers for cell toxicity and dysfunction.
Authors: Sebastian Mueller; Carlo Krupp; Sven Schnichels; Johanna Hofmann; Martin Spitzer; Karl Ulrich Bartz-Schmidt; Peter Szurman; Kai Januschowski Journal: Graefes Arch Clin Exp Ophthalmol Date: 2019-02-20 Impact factor: 3.117
Authors: Charles E Granger; Qiang Yang; Hongxin Song; Kenichi Saito; Koji Nozato; Lisa R Latchney; Bianca T Leonard; Mina M Chung; David R Williams; Ethan A Rossi Journal: Invest Ophthalmol Vis Sci Date: 2018-12-03 Impact factor: 4.799