Literature DB >> 16018545

Site-directed mutagenesis using Pfu DNA polymerase and T4 DNA ligase.

Yair Adereth1, Kristen J Champion, Tien Hsu, Vincent Dammai.   

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Year:  2005        PMID： 16018545      PMCID： PMC2265434          DOI： 10.2144/05386BM03

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


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  6 in total

1.  A one-tube, two-step polymerase chain reaction-based site-directed mutagenesis method with simple identification of the mutated product.

Authors:  Zsolt B Nagy; Ferenc Felföldi; László Tamás; László G Puskás
Journal:  Anal Biochem       Date:  2004-01-15       Impact factor: 3.365

2.  Enzymatic inverse PCR: a restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis.

Authors:  W P Stemmer; S K Morris
Journal:  Biotechniques       Date:  1992-08       Impact factor: 1.993

3.  Versatile PCR-mediated insertion or deletion mutagenesis.

Authors:  Jehan Lee; Hye-Jin Lee; Myeong-Kyun Shin; Wang-Shick Ryu
Journal:  Biotechniques       Date:  2004-03       Impact factor: 1.993

4.  Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.

Authors:  Joyce Chiu; Paul E March; Ryan Lee; Daniel Tillett
Journal:  Nucleic Acids Res       Date:  2004-12-07       Impact factor: 16.971

5.  Site-directed mutagenesis.

Authors:  T M Ishii; P Zerr; X M Xia; C T Bond; J Maylie; J P Adelman
Journal:  Methods Enzymol       Date:  1998       Impact factor: 1.600

6.  The "megaprimer" method of site-directed mutagenesis.

Authors:  G Sarkar; S S Sommer
Journal:  Biotechniques       Date:  1990-04       Impact factor: 1.993
  6 in total
  15 in total

Review 1.  A discussion of molecular biology methods for protein engineering.

Authors:  Alexander Zawaira; Anil Pooran; Samantha Barichievy; Denis Chopera
Journal:  Mol Biotechnol       Date:  2012-05       Impact factor: 2.695

2.  Efficient site-directed saturation mutagenesis using degenerate oligonucleotides.

Authors:  David L Steffens; John G K Williams
Journal:  J Biomol Tech       Date:  2007-07

3.  An evolutionarily conserved nuclear export signal facilitates cytoplasmic localization of the Tbx5 transcription factor.

Authors:  Andre Kulisz; Hans-Georg Simon
Journal:  Mol Cell Biol       Date:  2007-12-26       Impact factor: 4.272

4.  Specific expression of BMP2/4 ortholog in biomineralizing tissues of corals and action on mouse BMP receptor.

Authors:  Didier Zoccola; Aurélie Moya; Guillaume E Béranger; Eric Tambutté; Denis Allemand; Georges F Carle; Sylvie Tambutté
Journal:  Mar Biotechnol (NY)       Date:  2008-09-16       Impact factor: 3.619

5.  Flexible Connectors between Capsomer Subunits that Regulate Capsid Assembly.

Authors:  Mary L Hasek; Joshua B Maurer; Roger W Hendrix; Robert L Duda
Journal:  J Mol Biol       Date:  2017-07-10       Impact factor: 5.469

6.  GCM2-Activating Mutations in Familial Isolated Hyperparathyroidism.

Authors:  Bin Guan; James M Welch; Julie C Sapp; Hua Ling; Yulong Li; Jennifer J Johnston; Electron Kebebew; Leslie G Biesecker; William F Simonds; Stephen J Marx; Sunita K Agarwal
Journal:  Am J Hum Genet       Date:  2016-10-13       Impact factor: 11.025

7.  Transient contacts on the exterior of the HK97 procapsid that are essential for capsid assembly.

Authors:  Dan-ju Tso; Roger W Hendrix; Robert L Duda
Journal:  J Mol Biol       Date:  2014-03-20       Impact factor: 5.469

8.  Correct Assembly of the Bacteriophage T5 Procapsid Requires Both the Maturation Protease and the Portal Complex.

Authors:  Alexis Huet; Robert L Duda; Roger W Hendrix; Pascale Boulanger; James F Conway
Journal:  J Mol Biol       Date:  2015-11-23       Impact factor: 5.469

9.  Pseudomonas putida F1 has multiple chemoreceptors with overlapping specificity for organic acids.

Authors:  Rebecca E Parales; Rita A Luu; Grischa Y Chen; Xianxian Liu; Victoria Wu; Pamela Lin; Jonathan G Hughes; Vasyl Nesteryuk; Juanito V Parales; Jayna L Ditty
Journal:  Microbiology       Date:  2013-04-25       Impact factor: 2.777

10.  Taxis of Pseudomonas putida F1 toward phenylacetic acid is mediated by the energy taxis receptor Aer2.

Authors:  Rita A Luu; Benjamin J Schneider; Christie C Ho; Vasyl Nesteryuk; Stacy E Ngwesse; Xianxian Liu; Juanito V Parales; Jayna L Ditty; Rebecca E Parales
Journal:  Appl Environ Microbiol       Date:  2013-02-01       Impact factor: 4.792
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