Literature DB >> 14690696

A one-tube, two-step polymerase chain reaction-based site-directed mutagenesis method with simple identification of the mutated product.

Zsolt B Nagy1, Ferenc Felföldi, László Tamás, László G Puskás.   

Abstract

Mesh：

Substances：

Year:  2004        PMID： 14690696     DOI： 10.1016/j.ab.2003.09.009

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


× No keyword cloud information.
  5 in total

1.  Site-directed mutagenesis using Pfu DNA polymerase and T4 DNA ligase.

Authors:  Yair Adereth; Kristen J Champion; Tien Hsu; Vincent Dammai
Journal:  Biotechniques       Date:  2005-06       Impact factor: 1.993

2.  Multiple site directed mutagenesis strategy based on total RNA and RT-PCR method.

Authors:  Zsolt B Nagy; Márk Gárdonyi; Attila Mészáros; Zoltán Varga-Orvos; Robert G Solomon; László Tamás
Journal:  Mol Biotechnol       Date:  2007-07-19       Impact factor: 2.695

3.  Efficient site-directed saturation mutagenesis using degenerate oligonucleotides.

Authors:  David L Steffens; John G K Williams
Journal:  J Biomol Tech       Date:  2007-07

4.  An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening.

Authors:  Bao-zhong Zhang; Xin Zhang; Xiao-ping An; Duo-liang Ran; Yu-sen Zhou; Jun Lu; Yi-gang Tong
Journal:  J Zhejiang Univ Sci B       Date:  2009-06       Impact factor: 3.066

5.  Long distance multiple-site directed plasmid mutagenesis by one-step PCR using non-overlapped primers.

Authors:  Yuling Bu; Hui Wang; Jing Li; Zhaoxin Zhang; Yinghe Hu; Zhiliang Xu
Journal:  Mol Biotechnol       Date:  2013-09       Impact factor: 2.695
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.