| Literature DB >> 16009429 |
Jonghoon Kang1, Myung Soog Lee, David G Gorenstein.
Abstract
A PCR method for uniform amplification of a random sequence DNA library is described. A combination of 1 M betaine and 5% DMSO improves the PCR amplification by increasing the ratio of full-length products to shortened products, which are a consequence of nonuniform amplification due to stable secondary structures in the templates. This method is expected to be beneficial for obtaining high-affinity aptamers with stable secondary structures.Entities:
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Year: 2005 PMID： 16009429 DOI： 10.1016/j.jbbm.2005.06.003
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X