| Literature DB >> 16000785 |
Bill Widner1, Régine Behr, Steve Von Dollen, Maria Tang, Tia Heu, Alan Sloma, Dave Sternberg, Paul L Deangelis, Paul H Weigel, Steve Brown.
Abstract
The hasA gene from Streptococcus equisimilis, which encodes the enzyme hyaluronan synthase, has been expressed in Bacillus subtilis, resulting in the production of hyaluronic acid (HA) in the 1-MDa range. Artificial operons were assembled and tested, all of which contain the hasA gene along with one or more genes encoding enzymes involved in the synthesis of the UDP-precursor sugars that are required for HA synthesis. It was determined that the production of UDP-glucuronic acid is limiting in B. subtilis and that overexpressing the hasA gene along with the endogenous tuaD gene is sufficient for high-level production of HA. In addition, the B. subtilis-derived material was shown to be secreted and of high quality, comparable to commercially available sources of HA.Entities:
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Year: 2005 PMID： 16000785 PMCID： PMC1168996 DOI： 10.1128/AEM.71.7.3747-3752.2005
Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792