OBJECTIVE: This study investigated the temporal changes in whole-blood and plasma glutathione in ICU patients with multiple organ failure. DESIGN AND SETTING: Prospective and descriptive pilot study performed in an ICU with eight beds at a university hospital. PATIENTS: Critically ill patients (n=11) with multiple organ failure and ICU stay of at least 6 days were consecutively included. Patients with chronic obstructive pulmonary disease (n=21) and healthy volunteers (n=10) were used as reference groups. MEASUREMENTS AND RESULTS: Whole-blood and plasma glutathione were measured every 72 h. Total glutathione and the reduced fraction were determined in whole blood. The oxidized fraction and the redox status were calculated from these values. In plasma only the total concentration was determined. Patients were studied for 6-15 days. Nutrition was supplied according to routines supplying basal needs including glutamine. Both total and reduced glutathione was found to be depleted in whole blood compared to the reference groups. Redox status indicated continuing oxidative stress. Plasma glutathione showed higher values in total concentrations than the reference groups. CONCLUSIONS: This study demonstrates that glutathione remains depleted in whole blood. This contrasts to what has previously been shown in skeletal muscle where a restitution of glutathione concentration is seen.
OBJECTIVE: This study investigated the temporal changes in whole-blood and plasma glutathione in ICU patients with multiple organ failure. DESIGN AND SETTING: Prospective and descriptive pilot study performed in an ICU with eight beds at a university hospital. PATIENTS: Critically illpatients (n=11) with multiple organ failure and ICU stay of at least 6 days were consecutively included. Patients with chronic obstructive pulmonary disease (n=21) and healthy volunteers (n=10) were used as reference groups. MEASUREMENTS AND RESULTS: Whole-blood and plasma glutathione were measured every 72 h. Total glutathione and the reduced fraction were determined in whole blood. The oxidized fraction and the redox status were calculated from these values. In plasma only the total concentration was determined. Patients were studied for 6-15 days. Nutrition was supplied according to routines supplying basal needs including glutamine. Both total and reduced glutathione was found to be depleted in whole blood compared to the reference groups. Redox status indicated continuing oxidative stress. Plasma glutathione showed higher values in total concentrations than the reference groups. CONCLUSIONS: This study demonstrates that glutathione remains depleted in whole blood. This contrasts to what has previously been shown in skeletal muscle where a restitution of glutathione concentration is seen.
Authors: Yong-Ming Yu; Colleen M Ryan; Zhe-Wei Fei; Xiao-Ming Lu; Leticia Castillo; John T Schultz; Ronald G Tompkins; Vernon R Young Journal: Am J Physiol Endocrinol Metab Date: 2002-02 Impact factor: 4.310
Authors: M Reid; A Badaloo; T Forrester; J F Morlese; M Frazer; W C Heird; F Jahoor Journal: Am J Physiol Endocrinol Metab Date: 2000-03 Impact factor: 4.310
Authors: David Brealey; Michael Brand; Iain Hargreaves; Simon Heales; John Land; Ryszard Smolenski; Nathan A Davies; Chris E Cooper; Mervyn Singer Journal: Lancet Date: 2002-07-20 Impact factor: 79.321
Authors: Peter Andrews; Elie Azoulay; Massimo Antonelli; Laurent Brochard; Christian Brun-Buisson; Geoffrey Dobb; Jean-Yves Fagon; Herwig Gerlach; Johan Groeneveld; Jordi Mancebo; Philipp Metnitz; Stefano Nava; Jerome Pugin; Michael Pinsky; Peter Radermacher; Christian Richard; Robert Tasker Journal: Intensive Care Med Date: 2006-02-18 Impact factor: 17.440
Authors: Menghua Luo; Concepcion Fernandez-Estivariz; Dean P Jones; Carolyn R Accardi; Birgit Alteheld; Niloofar Bazargan; Li Hao; Daniel P Griffith; Jeffrey B Blumberg; John R Galloway; Thomas R Ziegler Journal: Nutrition Date: 2008-01 Impact factor: 4.008
Authors: Menghua Luo; Niloofar Bazargan; Daniel P Griffith; Concepción F Estívariz; Lorraine M Leader; Kirk A Easley; Nicole M Daignault; Li Hao; Jon B Meddings; John R Galloway; Jeffrey B Blumberg; Dean P Jones; Thomas R Ziegler Journal: Clin Nutr Date: 2008-02-07 Impact factor: 7.324
Authors: Kevin Cheung; Alasdair Rathbone; Michel Melanson; Jessica Trier; Benjamin R Ritsma; Matti D Allen Journal: J Appl Physiol (1985) Date: 2021-03-18
Authors: Zhe-Wei Fei; Vernon R Young; Xiao-Ming Lu; Andrew B Rhodes; Ronald G Tompkins; Alan J Fischman; Yong-Ming Yu Journal: Burns Trauma Date: 2013-09-18