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Literature DB >> 25913023

A simple assay for glutathione in whole blood.

Lovemore Hakuna¹, Brandon Doughan, Jorge O Escobedo, Robert M Strongin.

Abstract

A method for detecting glutathione selectively in whole blood deposited on filter paper is described. GSH is fractionated from proteins, hemoglobin and other potentially interfering components and determined using a resorufin-acrylate fluorescent probe. The relative standard deviation is lower than 5% (n = 5). Recoveries of GSH from whole blood are between 94% and 108.6%.

Entities: Chemical Disease Gene Species

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Year: 2015 PMID： 25913023 PMCID： PMC4449837 DOI： 10.1039/c5an00345h

Source DB: PubMed Journal: Analyst ISSN： 0003-2654 Impact factor: 4.616

30 in total

1. Ultrarapid capillary electrophoresis method for the determination of reduced and oxidized glutathione in red blood cells.

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Journal: Electrophoresis Date: 2002-06 Impact factor: 3.535

2. Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method.

Authors: Irfan Rahman; Aruna Kode; Saibal K Biswas
Journal: Nat Protoc Date: 2006 Impact factor: 13.491

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Authors: Magdalena L Circu; Tak Yee Aw
Journal: Free Radic Res Date: 2008-08

Review 4. Parkinson's disease: mechanisms and models.

Authors: William Dauer; Serge Przedborski
Journal: Neuron Date: 2003-09-11 Impact factor: 17.173

Review 5. Glutathione metabolism during aging and in Alzheimer disease.

Authors: Honglei Liu; Hong Wang; Swapna Shenvi; Tory M Hagen; Rui-Ming Liu
Journal: Ann N Y Acad Sci Date: 2004-06 Impact factor: 5.691

Review 6. Glutathione: overview of its protective roles, measurement, and biosynthesis.

Authors: Henry Jay Forman; Hongqiao Zhang; Alessandra Rinna
Journal: Mol Aspects Med Date: 2008-08-30

7. Circulating and muscle glutathione turnover in human endotoxaemia.

Authors: Urban B Fläring; Christina Hebert; Jan Wernerman; Folke Hammarqvist; Olav E Rooyackers
Journal: Clin Sci (Lond) Date: 2009-09-01 Impact factor: 6.124

8. Inherited disorders affecting mitochondrial function are associated with glutathione deficiency and hypocitrullinemia.

Authors: Kondala R Atkuri; Tina M Cowan; Tony Kwan; Angelina Ng; Leonard A Herzenberg; Leonore A Herzenberg; Gregory M Enns
Journal: Proc Natl Acad Sci U S A Date: 2009-02-17 Impact factor: 11.205

9. Selective spectrofluorimetric determination of glutathione in clinical and biological samples using 1,3,5,7-tetramethyl-8-phenyl-(2-maleimide)-difluoroboradiaza-s-indacene.

Authors: Xiao-Feng Guo; Hong Wang; Yue-Hong Guo; Hua-Shan Zhang
Journal: Anal Chim Acta Date: 2008-11-20 Impact factor: 6.558

Review 10. Glutathione dysregulation and the etiology and progression of human diseases.

Authors: Nazzareno Ballatori; Suzanne M Krance; Sylvia Notenboom; Shujie Shi; Kim Tieu; Christine L Hammond
Journal: Biol Chem Date: 2009-03 Impact factor: 3.915

7 in total

Review 1. Fluorescent Probes with Multiple Binding Sites for the Discrimination of Cys, Hcy, and GSH.

Authors: Cai-Xia Yin; Kang-Ming Xiong; Fang-Jun Huo; James C Salamanca; Robert M Strongin
Journal: Angew Chem Int Ed Engl Date: 2017-09-22 Impact factor: 15.336

Review 2. Glutathione production by Saccharomyces cerevisiae: current state and perspectives.

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Journal: Appl Microbiol Biotechnol Date: 2022-02-19 Impact factor: 4.813

3. Extending the Scope of ¹H NMR-Based Blood Metabolomics for the Analysis of Labile Antioxidants: Reduced and Oxidized Glutathione.

Authors: G A Nagana Gowda; Vadim Pascua; Daniel Raftery
Journal: Anal Chem Date: 2021-10-27 Impact factor: 6.986