[Description of the new species Anopheles artemievi sp.n. (Diptera, Culicidae)].

The paper describes the new species Anopheles artemievi belonging to a complex of the species maculipennis that may play an important role in the transmission of malaria in both the Fergana area and the Fergana valley. In terms of the morphological signs of imagoes, larvae, and the composition of the egg exochorion, the new species is the twin of An. sacharovi Favre and An. mihrtinius Shingarev. According to the pattern of polytene chromosomal disks, the described type is identical to An. maculipennis Meigen. The structure of the second internal transcribed spacer (ITS2) of a cluster of ribosomal genes in An. artemievi is species-specific and, in its nucleotide composition, differs from that in related species. The sequence of ITS2 in the new species has been registered at the GeneBank under No. AJ849886 and it is 419 p.n. The length of ITS2 in An. maculipennis and A. martinius is equal to 422 p.n. (AY238435) and 447 p.n. (AJ849885), respectively. The sequences of ITS in An. artemievi and An. maculipennis are 91% homologous; those in An. artemievi and An. martinius are 87% homologous. The nucleotide composition of ITS2 in An. artemievi differs from that in An. maculipennis in three deletions, 1 insertion, and 18 point substitutions and from that in An. martinius in 12 insertions, 2 deletions, and 40 point substitutions. The standard-type series is as follows: Kyrghyzstan, Batkensk Region, Leileksky district, Alga settlement, a cowshed, 1 male-holotype, l female-allotype. The paratypes are 2 males, 6 females.