| Literature DB >> 15978945 |
Young Lim Choi1, Ryozo Moriuchi, Mitsujiro Osawa, Atsushi Iwama, Hideki Makishima, Tomoaki Wada, Hiroyuki Kisanuki, Ruri Kaneda, Jun Ota, Koji Koinuma, Madoka Ishikawa, Shuji Takada, Yoshihiro Yamashita, Kazuo Oshimi, Hiroyuki Mano.
Abstract
Aggressive natural killer cell leukemia (ANKL) is an intractable malignancy that is characterized by the outgrowth of NK cells. To identify transforming genes in ANKL, we constructed a retroviral cDNA expression library from an ANKL cell line KHYG-1. Infection of 3T3 cells with recombinant retroviruses yielded 33 transformed foci. Nucleotide sequencing of the DNA inserts recovered from these foci revealed that 31 of them encoded KRAS2 with a glycine-to-alanine mutation at codon 12. Mutation-specific PCR analysis indicated that the KRAS mutation was present only in KHYG-1 cells, not in another ANKL cell line or in clinical specimens (n=8).Entities:
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Year: 2005 PMID： 15978945 DOI： 10.1016/j.leukres.2005.02.002
Source DB: PubMed Journal: Leuk Res ISSN： 0145-2126 Impact factor: 3.156