Literature DB >> 15973420

Colony filtration blot: a new screening method for soluble protein expression in Escherichia coli.

Tobias Cornvik1, Sue-Li Dahlroth, Audur Magnusdottir, Maria Dolores Herman, Rosemarie Knaust, Monica Ekberg, Pär Nordlund.   

Abstract

The implementation of efficient technologies for the production of recombinant mammalian proteins remains an outstanding challenge in many structural and functional genomics programs. We have developed a new method for rapid identification of soluble protein expression in E. coli, based on a separation of soluble protein from inclusion bodies by a filtration step at the colony level. The colony filtration (CoFi) blot is very well suited to screen libraries, and in the present work we used it to screen a deletion mutagenesis library.

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Year:  2005        PMID： 15973420     DOI： 10.1038/nmeth767

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  22 in total

1.  Combinatorial Domain Hunting: An effective approach for the identification of soluble protein domains adaptable to high-throughput applications.

Authors:  Stefanie Reich; Loretto H Puckey; Caroline L Cheetham; Richard Harris; Ammar A E Ali; Uma Bhattacharyya; Kate Maclagan; Keith A Powell; Chrisostomos Prodromou; Laurence H Pearl; Paul C Driscoll; Renos Savva
Journal:  Protein Sci       Date:  2006-10       Impact factor: 6.725

2.  Engineering membrane protein overproduction in Escherichia coli.

Authors:  Daniel Martinez Molina; Tobias Cornvik; Said Eshaghi; Jesper Z Haeggström; Pär Nordlund; Marina Ignatushchenko Sabet
Journal:  Protein Sci       Date:  2008-02-27       Impact factor: 6.725

Review 3.  Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production.

Authors:  Claudia Ortega; Pablo Oppezzo; Agustín Correa
Journal:  Methods Mol Biol       Date:  2022

4.  Large-scale identification of membrane proteins with properties favorable for crystallization.

Authors:  Jared Kim; Allison Kagawa; Kellie Kurasaki; Niloufar Ataie; Il Kyu Cho; Qing X Li; Ho Leung Ng
Journal:  Protein Sci       Date:  2015-08-27       Impact factor: 6.725

5.  Rapid expression screening of eukaryotic membrane proteins in Pichia pastoris.

Authors:  Cory L Brooks; Melissa Morrison; M Joanne Lemieux
Journal:  Protein Sci       Date:  2013-02-21       Impact factor: 6.725

Review 6.  Library methods for structural biology of challenging proteins and their complexes.

Authors:  Darren J Hart; Geoffrey S Waldo
Journal:  Curr Opin Struct Biol       Date:  2013-04-17       Impact factor: 6.809

7.  Challenges associated with heterologous expression of Flavobacterium psychrophilum proteins in Escherichia coli.

Authors:  Devendra H Shah; Kenneth D Cain; Gregory D Wiens; Douglas R Call
Journal:  Mar Biotechnol (NY)       Date:  2008-06-13       Impact factor: 3.619

8.  Experimental mapping of soluble protein domains using a hierarchical approach.

Authors:  Jean-Denis Pedelacq; Hau B Nguyen; Stephanie Cabantous; Brian L Mark; Pawel Listwan; Carolyn Bell; Natasha Friedland; Meghan Lockard; Alexandre Faille; Lionel Mourey; Thomas C Terwilliger; Geoffrey S Waldo
Journal:  Nucleic Acids Res       Date:  2011-07-19       Impact factor: 16.971

9.  A high-throughput immobilized bead screen for stable proteins and multi-protein complexes.

Authors:  Meghan A Lockard; Pawel Listwan; Jean-Denis Pedelacq; Stéphanie Cabantous; Hau B Nguyen; Thomas C Terwilliger; Geoffrey S Waldo
Journal:  Protein Eng Des Sel       Date:  2011-06-03       Impact factor: 1.650

10.  A simple and efficient expression and purification system using two newly constructed vectors.

Authors:  Huanting Liu; James H Naismith
Journal:  Protein Expr Purif       Date:  2008-09-20       Impact factor: 1.650
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