| Literature DB >> 35089549 |
Claudia Ortega1, Pablo Oppezzo1, Agustín Correa2.
Abstract
Despite the importance of recombinant protein production in the academy and industrial fields, many issues concerning the expression of soluble and homogeneous products are still unsolved. Several strategies were developed to overcome these obstacles; however, at present, there is no magic bullet that can be applied for all cases. Indeed, several key expression parameters need to be evaluated for each protein. Among the different hosts for protein expression, Escherichia coli is by far the most widely used. In this chapter, we review many of the different tools employed to circumvent protein insolubility problems.Entities:
Keywords: Directed evolution; E. coli; High-throughput screening; Inclusion bodies; Protein expression; Recombinant proteins
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Year: 2022 PMID： 35089549 DOI： 10.1007/978-1-0716-1859-2_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745