Literature DB >> 15965750

Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part III. Hydrogels as carriers for immobilization of proteins.

J Michálek1, M Prádný, A Artyukhov, M Slouf, K Smetana.   

Abstract

Four series of macroporous hydrogels based on crosslinked copolymers of 2-hydroxyethyl methacrylate (HEMA)-sodium methacrylate (MANa), copolymer HEMA-[2-(methacryloyloxy)ethyl]trimethylammonium chloride (MOETACl), terpolymer HEMA-MANa-MOETACl and on a polyelectrolyte complex were used as carriers for immobilization of proteins, chicken egg white albumin and avidin. The adsorption capacity of the hydrogels for the two proteins, kinetics and pH dependence of albumin adsorption and desorption were studied. The morphology of the hydrogels with and without immobilized albumin was studied by low-vacuum scanning electron microscopy.

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Year:  2005        PMID： 15965750     DOI： 10.1007/s10856-005-2617-2

Source DB:  PubMed          Journal:  J Mater Sci Mater Med        ISSN： 0957-4530            Impact factor:   3.896


  8 in total

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Journal:  Nature       Date:  2001-11-01       Impact factor: 49.962

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Authors:  Jirí Labský; Barbora Dvoránková; Karel Smetana; Zuzana Holíková; Ludomír Broz; Hans-Joachim Gabius
Journal:  Biomaterials       Date:  2003-02       Impact factor: 12.479

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Journal:  Biochem J       Date:  1965-03       Impact factor: 3.857

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Journal:  J Biomed Mater Res       Date:  1990-04

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Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

Review 6.  Pluripotent stem cells--model of embryonic development, tool for gene targeting, and basis of cell therapy.

Authors:  Katja Prelle; Nicola Zink; Eckhard Wolf
Journal:  Anat Histol Embryol       Date:  2002-06       Impact factor: 1.114

Review 7.  Cell biology of hydrogels.

Authors:  K Smetana
Journal:  Biomaterials       Date:  1993-11       Impact factor: 12.479

8.  Compositional and structural heterogeneity of avidin glycopeptides.

Authors:  R C Bruch; H B White
Journal:  Biochemistry       Date:  1982-10-12       Impact factor: 3.162
  8 in total
  5 in total

1.  Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part 5: hydrolytically degradable materials.

Authors:  Martin Prádný; Jirí Michálek; Petr Lesný; Ales Hejcl; Jirí Vacík; Miroslav Slouf; Eva Syková
Journal:  J Mater Sci Mater Med       Date:  2006-12       Impact factor: 3.896

2.  Surface-modified P(HEMA-co-MAA) nanogel carriers for oral vaccine delivery: design, characterization, and in vitro targeting evaluation.

Authors:  Matilde Durán-Lobato; Brenda Carrillo-Conde; Yasmine Khairandish; Nicholas A Peppas
Journal:  Biomacromolecules       Date:  2014-07-02       Impact factor: 6.988

3.  Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part 4: growth of rat bone marrow stromal cells in three-dimensional hydrogels with positive and negative surface charges and in polyelectrolyte complexes.

Authors:  P Lesný; M Prádný; P Jendelová; J Michálek; J Vacík; E Syková
Journal:  J Mater Sci Mater Med       Date:  2006-09       Impact factor: 3.896

4.  Real time in vitro studies of doxorubicin release from PHEMA nanoparticles.

Authors:  Raje Chouhan; Ak Bajpai
Journal:  J Nanobiotechnology       Date:  2009-10-20       Impact factor: 10.435

5.  Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part 6: 3D hydrogels with positive and negative surface charges and polyelectrolyte complexes in spinal cord injury repair.

Authors:  A Hejcl; P Lesný; M Prádný; J Sedý; J Zámecník; P Jendelová; J Michálek; E Syková
Journal:  J Mater Sci Mater Med       Date:  2009-02-28       Impact factor: 3.896
  5 in total

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