BACKGROUND/ METHODS: We compared the diagnostic yield of a real time polymerase chain reaction (PCR) assay in cerebrospinal fluid (CSF) samples with conventional microbiological techniques for the diagnosis of neurobrucellosis. Following amplification of a 223 bp sequence specific for Brucella genus, melting curve analysis was performed to verify the specificity of the PCR products. RESULTS: All six patients with neurobrucellosis (three meningitis and three meningoencephalitis) had a positive real time PCR assay, whereas CSF cultures and Wright seroagglutination tests were positive in only two and four cases, respectively. Brucella specific amplicons were easily demonstrated by their characteristic melting temperature in all the real time PCR assays. CONCLUSION: LightCycler based real time PCR assay in CSF samples is more rapid and sensitive than conventional microbiological tests. This technique could be useful for the rapid diagnosis of neurobrucellosis.
BACKGROUND/ METHODS: We compared the diagnostic yield of a real time polymerase chain reaction (PCR) assay in cerebrospinal fluid (CSF) samples with conventional microbiological techniques for the diagnosis of neurobrucellosis. Following amplification of a 223 bp sequence specific for Brucella genus, melting curve analysis was performed to verify the specificity of the PCR products. RESULTS: All six patients with neurobrucellosis (three meningitis and three meningoencephalitis) had a positive real time PCR assay, whereas CSF cultures and Wright seroagglutination tests were positive in only two and four cases, respectively. Brucella specific amplicons were easily demonstrated by their characteristic melting temperature in all the real time PCR assays. CONCLUSION: LightCycler based real time PCR assay in CSF samples is more rapid and sensitive than conventional microbiological tests. This technique could be useful for the rapid diagnosis of neurobrucellosis.
Authors: Daniel Tena; Alejandro González-Praetorius; Alberto López-Alonso; José Luis Peña; María Teresa Pérez-Pomata; Julia Bisquert Journal: Eur J Pediatr Date: 2006-05-12 Impact factor: 3.183
Authors: Maria Isabel Queipo-Ortuño; Juan De Dios Colmenero; Manuel Macias; Maria Jose Bravo; Pilar Morata Journal: Clin Vaccine Immunol Date: 2007-12-12
Authors: Vladimira Hinić; Isabelle Brodard; Andreas Thomann; Milena Holub; Raymond Miserez; Carlos Abril Journal: BMC Vet Res Date: 2009-07-14 Impact factor: 2.741