Literature DB >> 15963773

Crystallization of bacteriorhodopsin solubilized by a tripod amphiphile.

Michael J Theisen1, Terra B Potocky, D Tyler McQuade, Samuel H Gellman, Mark L Chiu.   

Abstract

Bacteriorhodopsin (bR) is solubilized efficiently as a monomer by a novel surfactant, a tripod amphiphile (TPA), which permits the formation of purple hexagonal bR crystals under several conditions. The crystals, although small, diffract to 2.5 A resolution using synchrotron radiation. TPA may be useful for the solubilization, purification, and crystallization of other membrane proteins.

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Year:  2005        PMID: 15963773     DOI: 10.1016/j.bbapap.2005.04.011

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


  7 in total

1.  Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins.

Authors:  Hak Jun Kim; Stanley C Howell; Wade D Van Horn; Young Ho Jeon; Charles R Sanders
Journal:  Prog Nucl Magn Reson Spectrosc       Date:  2009-11-01       Impact factor: 9.795

2.  Steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins.

Authors:  Sung Chang Lee; Brad C Bennett; Wen-Xu Hong; Yu Fu; Kent A Baker; Julien Marcoux; Carol V Robinson; Andrew B Ward; James R Halpert; Raymond C Stevens; Charles David Stout; Mark J Yeager; Qinghai Zhang
Journal:  Proc Natl Acad Sci U S A       Date:  2013-03-11       Impact factor: 11.205

3.  Crystallographic characterization of N-oxide tripod amphiphiles.

Authors:  Pil Seok Chae; Ilia A Guzei; Samuel H Gellman
Journal:  J Am Chem Soc       Date:  2010-02-17       Impact factor: 15.419

4.  Glycotripod amphiphiles for solubilization and stabilization of a membrane-protein superassembly: importance of branching in the hydrophilic portion.

Authors:  Pil S Chae; Marc J Wander; Aaron P Bowling; Philip D Laible; Samuel H Gellman
Journal:  Chembiochem       Date:  2008-07-21       Impact factor: 3.164

5.  Tripod Amphiphiles for Membrane Protein Manipulation.

Authors:  Pil Seok Chae; Philip D Laible; Samuel H Gellman
Journal:  Mol Biosyst       Date:  2010

6.  Hydrophobic variants of ganglio-tripod amphiphiles for membrane protein manipulation.

Authors:  Pil Seok Chae; Kyung Ho Cho; Marc J Wander; Hyoung Eun Bae; Samuel H Gellman; Philip D Laible
Journal:  Biochim Biophys Acta       Date:  2013-09-21

7.  Designer lipid-like peptides: a class of detergents for studying functional olfactory receptors using commercial cell-free systems.

Authors:  Karolina Corin; Philipp Baaske; Deepali B Ravel; Junyao Song; Emily Brown; Xiaoqiang Wang; Christoph J Wienken; Moran Jerabek-Willemsen; Stefan Duhr; Yuan Luo; Dieter Braun; Shuguang Zhang
Journal:  PLoS One       Date:  2011-11-23       Impact factor: 3.240

  7 in total

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