Literature DB >> 15954761

Conformational change in unsolvated Trp-cage protein probed by fluorescence.

Anthony T Iavarone1, Joel H Parks.   

Abstract

We report the first direct measurements of the unfolding of a protein, Trp-cage, in the gas phase using laser-induced fluorescence of protein ions in a heated quadrupole ion trap. The changes in enthalpy and entropy associated with the observed conformational change are obtained by fitting a two-state model of protein unfolding to the fluorescence intensities plotted versus temperature. The enthalpy and entropy changes for the 2+ and 3+ charge states are greater than the values measured in solution and depend on charge state.

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Year:  2005        PMID： 15954761     DOI： 10.1021/ja051788u

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  13 in total

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Authors:  Changgeng Feng; Juliette J Commodore; Carolyn J Cassady
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2.  Effect of buffer gas on the fluorescence yield of trapped gas-phase ions.

Authors:  Maxim Dashtiev; Renato Zenobi
Journal:  J Am Soc Mass Spectrom       Date:  2006-04-17       Impact factor: 3.109

3.  Which electrospray-based ionization method best reflects protein-ligand interactions found in solution? a comparison of ESI, nanoESI, and ESSI for the determination of dissociation constants with mass spectrometry.

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Journal:  J Am Soc Mass Spectrom       Date:  2007-11-19       Impact factor: 3.109

4.  Exploring fluorescence and fragmentation of ions produced by electrospray ionization in ultrahigh vacuum.

Authors:  Konstantin Chingin; Huanwen Chen; Gerardo Gamez; Renato Zenobi
Journal:  J Am Soc Mass Spectrom       Date:  2009-05-29       Impact factor: 3.109

5.  Fluorescence and photodissociation of rhodamine 575 cations in a quadrupole ion trap.

Authors:  Nicholas A Sassin; Stephanie C Everhart; Beni B Dangi; Kent M Ervin; Joseph I Cline
Journal:  J Am Soc Mass Spectrom       Date:  2008-09-18       Impact factor: 3.109

6.  Gas-phase fluorescence excitation and emission spectroscopy of three xanthene dyes (rhodamine 575, rhodamine 590 and rhodamine 6G) in a quadrupole ion trap mass spectrometer.

Authors:  Matthew W Forbes; Rebecca A Jockusch
Journal:  J Am Soc Mass Spectrom       Date:  2011-01-28       Impact factor: 3.109

7.  Fluorescence lifetime probe of biomolecular conformations.

Authors:  Xiangguo Shi; Joel H Parks
Journal:  J Am Soc Mass Spectrom       Date:  2010-01-25       Impact factor: 3.109

8.  Structural Effects of Solvation by 18-Crown-6 on Gaseous Peptides and TrpCage after Electrospray Ionization.

Authors:  James G Bonner; Nathan G Hendricks; Ryan R Julian
Journal:  J Am Soc Mass Spectrom       Date:  2016-08-09       Impact factor: 3.109

9.  On the zwitterionic nature of gas-phase peptides and protein ions.

Authors:  Roberto Marchese; Rita Grandori; Paolo Carloni; Simone Raugei
Journal:  PLoS Comput Biol       Date:  2010-05-06       Impact factor: 4.475

10.  Temperature and urea induced denaturation of the TRP-cage mini protein TC5b: A simulation study consistent with experimental observations.

Authors:  Z Gattin; S Riniker; P J Hore; K H Mok; W F van Gunsteren
Journal:  Protein Sci       Date:  2009-10       Impact factor: 6.725
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