| Literature DB >> 1594448 |
M Lechner1, B Frey, F Laue, J Anton-Botella, C L Smith, W Ankenbauer, G G Schmitz.
Abstract
A novel class-II restriction endonuclease designated SwaI was purified from Staphylococcus warneri. This enzyme cleaves adenovirus 2 DNA, SV40 DNA and M13mp7 at one site each, but does not cleave lambda, PhiX174, pBR322 or pBR328 DNA. SwaI recognizes the octanucleotide sequence 5'-ATTTAAAT-3', cleaving in the center of the recognition sequence creating blunt ended DNA fragments. SwaI was used to digest chromosomal DNA from various microorganisms and human cells.Entities:
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Year: 1992 PMID: 1594448 PMCID: PMC312344 DOI: 10.1093/nar/20.9.2293
Source DB: PubMed Journal: Nucleic Acids Res ISSN: 0305-1048 Impact factor: 16.971