| Literature DB >> 15939503 |
Ana Rute Neves1, Wietske A Pool, Jan Kok, Oscar P Kuipers, Helena Santos.
Abstract
The wide application of lactic acid bacteria in the production of fermented foods depends to a great extent on the unique features of sugar metabolism in these organisms. The relative metabolic simplicity and the availability of genetic tools made Lactococcus lactis the organism of choice to gain insight into metabolic and regulatory networks. In vivo nuclear magnetic resonance has proven a very useful technique to monitor non-invasively the dynamics of intracellular metabolite and co-factor pools following a glucose pulse. Examples of the application of this methodology to identify metabolic bottlenecks and regulatory sites are presented. The use of this information to direct metabolic engineering strategies is illustrated.Entities:
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Year: 2005 PMID： 15939503 DOI： 10.1016/j.femsre.2005.04.005
Source DB: PubMed Journal: FEMS Microbiol Rev ISSN： 0168-6445 Impact factor: 16.408