OBJECTIVE: To examine the physiologic role of natural killer T (NKT) cells bearing V(alpha)14 T cell receptor (TCR) in the pathogenesis of collagen-induced arthritis (CIA) and antibody-induced arthritis in mice. METHODS: NKT cells were stained with alpha-galactosylceramide-loaded CD1 dimer, and then assessed using flow cytometry. CIA was induced in mice by immunization on days 0 and 21 with type II collagen (CII) emulsified with an equal volume of Freund's complete adjuvant. Anti-CII antibodies were measured by enzyme-linked immunosorbent assay. For antibody-induced arthritis, mice were injected with anti-CII monoclonal antibodies (mAb) followed by lipopolysaccharide, or with serum from KRN TCR-transgenic mice crossed with nonobese diabetic mice (K/BxN). The severity of arthritis was monitored with a macroscopic scoring system. RESULTS: The number of NKT cells increased in the liver at the peak of the clinical course of CIA. Administration of anti-CD1 mAb inhibited development of CIA. The severity of CIA in NKT cell-deficient mice was reduced compared with that in wild-type mice. The IgG1:IgG2a ratio of anti-CII was elevated and production of interleukin-10 from draining lymph node cells was increased in NKT cell-deficient mice. NKT cell-deficient mice were significantly less susceptible to antibody-induced arthritis. CONCLUSION: NKT cells contribute to the pathogenesis of arthritis by enhancing autoantibody-mediated inflammation. NKT cells also contribute to the disease process in a deleterious way, due, at least in part, to the alteration of the Th1/Th2 balance in T cell response to CII.
OBJECTIVE: To examine the physiologic role of natural killer T (NKT) cells bearing V(alpha)14 T cell receptor (TCR) in the pathogenesis of collagen-induced arthritis (CIA) and antibody-induced arthritis in mice. METHODS: NKT cells were stained with alpha-galactosylceramide-loaded CD1 dimer, and then assessed using flow cytometry. CIA was induced in mice by immunization on days 0 and 21 with type II collagen (CII) emulsified with an equal volume of Freund's complete adjuvant. Anti-CII antibodies were measured by enzyme-linked immunosorbent assay. For antibody-induced arthritis, mice were injected with anti-CII monoclonal antibodies (mAb) followed by lipopolysaccharide, or with serum from KRN TCR-transgenic mice crossed with nonobese diabeticmice (K/BxN). The severity of arthritis was monitored with a macroscopic scoring system. RESULTS: The number of NKT cells increased in the liver at the peak of the clinical course of CIA. Administration of anti-CD1 mAb inhibited development of CIA. The severity of CIA in NKT cell-deficient mice was reduced compared with that in wild-type mice. The IgG1:IgG2a ratio of anti-CII was elevated and production of interleukin-10 from draining lymph node cells was increased in NKT cell-deficient mice. NKT cell-deficient mice were significantly less susceptible to antibody-induced arthritis. CONCLUSION: NKT cells contribute to the pathogenesis of arthritis by enhancing autoantibody-mediated inflammation. NKT cells also contribute to the disease process in a deleterious way, due, at least in part, to the alteration of the Th1/Th2 balance in T cell response to CII.
Authors: Jonathan M Coquet; Sumone Chakravarti; Konstantinos Kyparissoudis; Finlay W McNab; Lauren A Pitt; Brent S McKenzie; Stuart P Berzins; Mark J Smyth; Dale I Godfrey Journal: Proc Natl Acad Sci U S A Date: 2008-08-06 Impact factor: 11.205
Authors: Y Yoshiga; D Goto; S Segawa; M Horikoshi; T Hayashi; I Matsumoto; S Ito; M Taniguchi; T Sumida Journal: Clin Exp Immunol Date: 2011-03-10 Impact factor: 4.330