Literature DB >> 15930621

Crystallization and preliminary X-ray analysis of strictosidine synthase and its complex with the substrate tryptamine.

Juergen Koepke1, Xueyan Ma, Günter Fritzsch, Hartmut Michel, Joachim Stöckigt.   

Abstract

Strictosidine synthase (STR1) is a central enzyme that participates in the biosynthesis of almost all plant monoterpenoid indole alkaloids. After heterologous expression in Escherichia coli, crystals of STR1 and its substrate complex with tryptamine were obtained by the hanging-drop technique at 302-304 K with potassium sodium tartrate tetrahydrate as precipitant. All crystals belong to space group R3. The native STR1 crystals diffract to 2.95 A and have unit-cell parameters a = b = 150.3, c = 122.4 A. The tryptamine complex crystals diffract to 2.38 A, with unit-cell parameters a = b = 147.3, c = 122.3 A.

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Year:  2005        PMID： 15930621     DOI： 10.1107/S0907444904029348

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  7 in total

1.  Heterologous expression, purification, crystallization and preliminary X-ray analysis of raucaffricine glucosidase, a plant enzyme specifically involved in Rauvolfia alkaloid biosynthesis.

Authors:  Martin Ruppert; Santosh Panjikar; Leif Barleben; Joachim Stöckigt
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-02-24

2.  The structure of Rauvolfia serpentina strictosidine synthase is a novel six-bladed beta-propeller fold in plant proteins.

Authors:  Xueyan Ma; Santosh Panjikar; Juergen Koepke; Elke Loris; Joachim Stöckigt
Journal:  Plant Cell       Date:  2006-03-10       Impact factor: 11.277

3.  Expression, purification, crystallization and preliminary X-ray analysis of perakine reductase, a new member of the aldo-keto reductase enzyme superfamily from higher plants.

Authors:  Cindy Rosenthal; Uwe Mueller; Santosh Panjikar; Lianli Sun; Martin Ruppert; Yu Zhao; Joachim Stöckigt
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-11-30

4.  Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system.

Authors:  Martin Ruppert; Jörn Woll; Anatoli Giritch; Ezzat Genady; Xueyan Ma; Joachim Stöckigt
Journal:  Planta       Date:  2005-08-18       Impact factor: 4.116

5.  Molecular architecture of strictosidine glucosidase: the gateway to the biosynthesis of the monoterpenoid indole alkaloid family.

Authors:  Leif Barleben; Santosh Panjikar; Martin Ruppert; Juergen Koepke; Joachim Stöckigt
Journal:  Plant Cell       Date:  2007-09-21       Impact factor: 11.277

6.  LAP3, a novel plant protein required for pollen development, is essential for proper exine formation.

Authors:  Anna A Dobritsa; Shuh-Ichi Nishikawa; Daphne Preuss; Ewa Urbanczyk-Wochniak; Lloyd W Sumner; Adam Hammond; Ann L Carlson; Robert J Swanson
Journal:  Sex Plant Reprod       Date:  2009-06-11

7.  Inverted Binding of Non-natural Substrates in Strictosidine Synthase Leads to a Switch of Stereochemical Outcome in Enzyme-Catalyzed Pictet-Spengler Reactions.

Authors:  Elisabeth Eger; Adam Simon; Mahima Sharma; Song Yang; Willem B Breukelaar; Gideon Grogan; K N Houk; Wolfgang Kroutil
Journal:  J Am Chem Soc       Date:  2020-01-07       Impact factor: 15.419
  7 in total

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